LOCUS       sequence-65112-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Datsenko KA, Wanner BL
  TITLE     One-step inactivation of chromosomal genes in Escherichia coli K-12 
            using PCR products.
  JOURNAL   Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5.
  PUBMED    10829079
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2000-06-6"; volume: "97(12)"; pages: 
            "6640-5"
COMMENT     Sequence Label: pKD3
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    83..116
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        222..324
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(391..410)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ttttgagtga cacaggaaca cttaacggct gacatgggaa ttagccatgg tccatatgaa
       61 tatcctcctt agttcctatt ccgaagttcc tattctctag aaagtatagg aacttcggcg
      121 cgcctacctg tgacggaaga tcacttcgca gaataaataa atcctggtgt ccctgttgat
      181 accgggaagc cctgggccaa cttttggcga aaatgagacg ttgatcggca cgtaagaggt
      241 tccaactttc accataatga aataagatca ctaccgggcg tattttttga gttgtcgaga
      301 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
      361 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
      421 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
      481 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
      541 ttacgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
      601 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
      661 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
      721 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt
      781 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttt
//
