LOCUS       sequence-65113-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Datsenko KA, Wanner BL
  TITLE     One-step inactivation of chromosomal genes in Escherichia coli K-12 
            using PCR products.
  JOURNAL   Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5.
  PUBMED    10829079
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2000-06-6"; volume: "97(12)"; pages: 
            "6640-5"
COMMENT     Sequence Label: pKD4
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    77..110
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(412..431)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gtgacacagg aacacttaac ggctgacatg ggaattagcc atggtccata tgaatatcct
       61 ccttagttcc tattccgaag ttcctattct ctagaaagta taggaacttc agagcgcttt
      121 tgaagctggg gtgggcgaag aactccagca tgagatcccc gcgctggagg atcatccagc
      181 cggcgtcccg gaaaacgatt ccgaagccca acctttcata gaaggcggcg gtggaatcga
      241 aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc
      301 tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat
      361 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg
      421 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa
      481 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac
      541 gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc
      601 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt
      661 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag
      721 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg
      781 agatgacagg agatcctgcc ccggcacttc gcccaat
//
