LOCUS       sequence-65128-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Murakami KS
  TITLE     X-ray Crystal Structure of Escherichia coli RNA Polymerase sigma70 
            Holoenzyme.
  JOURNAL   J Biol Chem. 2013 Mar 29;288(13):9126-34. doi: 
            10.1074/jbc.M112.430900. Epub 2013 Feb 6.
  PUBMED    23389035
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.430900"; journalName: "J Biol Chem"; date: 
            "2013-03-29- 29"; volume: "288"; issue: "13"; pages: "9126-34"
COMMENT     Sequence Label: pGEMABC
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..58
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gcttcctttc gggctttgtt agcagccgga tcaagctggg atttaggtga cactatagga
       61 tccggattct gacaaatgct ctttccctaa actcccccca taaaaaaacc cgccgaagcg
      121 ggtttttacg ttatttgcgg attaacgatt actcgttatc agaaccgccc agacctgcgt
      181 tcagcagttc tgccaggctg gcagatgcgt cttctgcagt cacctgcggt gcagccggag
      241 cttcacccgc agcacggcga cgcatacgat cctggtggta cgcgtaaccg gtacctgccg
      301 ggatcagacg acccacgata acgttctctt tcaggccgcg cagttcgtcg cgtttgcccg
      361 caacggctgc ttcggtcagc acgcgagtgg tctcctggaa cgatgccgcg gagatgaagg
      421 actcggttgc cagagacgct ttggtgatac ccagcagatc gcgggagtaa gttgcaccca
      481 ctttgccgtt cgcttccagt tcgcggtttg cgatcttgac gcgagagtat tcaacctgtt
      541 cgccttccag gaagtcggag ctacccgcgt taacgatggt agctttacgc agcatctgac
      601 gaacgataac ttcgatgtgt ttatcgttaa tcttaacgcc ctgcagacgg tatacgtcct
      661 gtacttcgtt aacgatgtaa cgagtaacag catgaacacc acgcagacgc agaatgtcgt
      721 gcggcgcttc cggaccgtcg gaaattacgt caccacgttc tacacgttca ccttcgaaca
      781 cgttgagctg acgccatttc ggaatcatct cttcgtacgg atcgctaccg tctaccgggg
      841 tgataaccag acgacgttta cctttggttt ctttaccgaa ggaaacgata ccgctgattt
      901 cagccaggat tgccggctct ttcggacgac
//
