LOCUS       sequence-65134-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Molyneaux BJ, Arlotta P, Fame RM, MacDonald JL, MacQuarrie KL, 
            Macklis JD
  TITLE     Novel subtype-specific genes identify distinct subpopulations of 
            callosal projection neurons.
  JOURNAL   J Neurosci. 2009 Sep 30;29(39):12343-54. doi: 
            10.1523/JNEUROSCI.6108-08.2009.
  PUBMED    19793993
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.6108-08"; journalName: "J Neurosci"; date: 
            "2009-09-30- 30"; volume: "29"; issue: "39"; pages: "12343-54"
COMMENT     Sequence Label: pCRII-Topo Frmd4b
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..23
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5..22
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(775..794)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(776..794)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(801..818)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(801..817)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(810..832)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 agctatttag gtgacactat agaatactca agctatgcat caagcttggt accgagctcg
       61 gatccactag taacggccgc cagtgtgctg gaattcgccc tttcctgcag ctcggagtaa
      121 attctttcct gtcaatctcg gcttgtcact ttgtgactcg gatttgcttc tggagttcag
      181 gtcgtaggct tcattttcta cggttcacca ctttaaggca cacaattcat ctacggtgat
      241 gtgggttggc tttgtcctct ggggtccttt ctgttagcat ctccagacac tttttctgga
      301 taaacttgcc acctcgagcc caccgttcag gtgacaggca aggtcaccag tctgaacagt
      361 cagtgttggg aagcacaaca ggaattggtg gcatgtacct taagaacatt ctccaagcag
      421 agctggccag tgacttcatt tcctctagtc agtgactgtt aagttatagt tacaggatgc
      481 tatagcaatc agaagactag aatccatatt agggctctta tgaagaacat tgctttgcaa
      541 tagcctaaaa catctacact cattcagaac ttcatgaaaa tctaacttct cttcaagtaa
      601 gtttccaata ctgtctaaac tgtcctcatc tctttgtaga aacggtgagt tttctgtcag
      661 ctcacaagtt caaactctgt ggagataagc cacgattcag gagctaaggg cgaattctgc
      721 agatatccat cacactggcg gccgctcgag catgcatcta gagggcccaa ttcgccctat
      781 agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg gga
//
