LOCUS       sequence-65135-e         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  TITLE     Yeast Secretion Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRS316-4M5.3
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             134..157
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ccggatcatg aaggttttga ttgtcttgtt ggctatcttc gctgctttgc ccttggctct
       61 agctcaaccg gttatttcta ctaccgtcgg ttccgctgca gaaggctctt tggacaagag
      121 agaagctcgg ccggactaca aggacgacga tgacaaggct agcgacgtcg ttatgactca
      181 aacaccacta tcacttcctg ttagtctagg tgatcaagcc tccatctctt gcagatctag
      241 tcagagcctc gtacacagta atggaaacac ctatttacgt tggtacctgc agaagccagg
      301 ccagtctcca aaggtcctga tctacaaagt ttccaaccga gtttctgggg tcccagacag
      361 gttcagtggc agtggatcag ggacagattt cacactcaag atcaacagag tggaggctga
      421 ggatctggga gtttatttct gctctcaaag tacacatgtt ccgtggacgt tcggtggagg
      481 caccaagctt gaaattaagt cctctgctga tgatgctaag aaggatgctg ctaagaagga
      541 tgatgctaag aaagatgatg ctaagaaaga tggtggcgtc aaactggatg agactggagg
      601 aggcttggtg caacctgggg gggccatgaa actctcctgt gttacctctg gattcacttt
      661 tggtcactac tggatgaact gggtccgcca gtctccagag aaaggactgg agtgggtagc
      721 acaatttaga aacaaacctt ataattatga aacatattat tcagattctg tgaaaggcag
      781 attcaccatc tcaagagatg attccaaaag tagtgtctat ctgcaaatga acaacttaag
      841 agttgaagac acgggtatct attactgtac gggtgcttcc tatggtatgg aatacttggg
      901 tcaaggaacc tcagtcaccg tctcctgata acaaaagctt atttctgaag aaga
//
