LOCUS       sequence-65139-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  TITLE     Macklis lab in situ probes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCRII-Topo Couptf1
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..23
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5..22
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(799..818)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(800..818)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(825..842)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(825..841)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(834..856)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 agctatttag gtgacactat agaatactca agctatgcat caagcttggt accgagctcg
       61 gatccactag taacggccgc cagtgtgctg gaattcgccc ttttgaagaa cagcctcgac
      121 aacatatatc gcactcatta taagaagcaa agggttatat caaaaattat tagtatagaa
      181 tcacaggaaa tctggtttgg aaccagaaaa aatacaaaac agatatagat acatagacac
      241 aggacaaatt ttttataatt atttggaaaa tgttatttcc ccctaattct tgttcatttt
      301 tgtttatgcg catacgatgt ttaaatttta caaaaaatga aaataaacac tagtatttta
      361 caaaatgaat aacaatgttc agtgtatgtt tctacatagg attcagtcca cttccatatg
      421 agtagttttc tgtacagaat atatccacat ccatccacaa taaatcctgg aaggtccatc
      481 tattttatta aatttctcat ggtcctctct tccaaatcgt tctttttttt tttttttttt
      541 tcattttctc accagacacg aggtcctttt tctttccttt ccaatgtact tacagatcag
      601 atcctaaatg agtctccttt tctcctggtt tgcagctcag gttgcattcc tgcagagtgg
      661 gctgctcttg ttcccagccc tcctcccgtc tgcctggcct gcctggtcca tcataccagc
      721 atccccaaaa agggcgaatt ctgcagatat ccatcacact ggcggccgct cgagcatgca
      781 tctagagggc ccaattcgcc ctatagtgag tcgtattaca attcactggc cgtcgtttta
      841 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
      901 cctttcgcca gctgg
//
