LOCUS       sequence-65143-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  TITLE     Macklis lab in situ probes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCRII-Topo Hnrp
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(504..523)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(505..523)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(530..547)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(530..546)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(539..561)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(774..793)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agctatttgg tgacactata gaatactcaa gctatgcatc aagcttggta ccgagctcgg
       61 atccactagt aacggccgcc agtgtgctgg aattcgccct tttctgctgc cacaaagact
      121 gtaaagtgct tcatttcaac agctgaggca agccaagtga tcattaataa agcttttctt
      181 gcttccttca gtggtgttgg tagtaaaatg gaaggtgtct tgctgcaggt aactaatgaa
      241 gaagtggtca accacagagt cttcaagaaa taagaaattc tgtaccatct gaaagtagtt
      301 cttgttggtg ccttcattta agaagcactt ttaagaaaag ggaacgtttt ctgatttaaa
      361 acttcattta gtacaggttc ttaataaaat tacaaaaatg ctgattgtaa agggtaacat
      421 caaattagca aaggaagggc gaattctgca gatatccatc acactggcgg ccgctcgagc
      481 atgcatctag agggcccaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg
      541 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
      601 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
      661 agttgcgcag cctgaatggc gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg
      721 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
      781 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
      841 ggggctccct tta
//
