LOCUS       sequence-65144-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  TITLE     Macklis lab in situ probes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCRII-Topo Lhx2
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..23
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5..22
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(578..597)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(579..597)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(604..621)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(604..620)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(613..635)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(848..867)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agctatttag gtgacactat agaatactca agctatgcat caagcttggt accgagctcg
       61 gatccactag taacggccgc cagtgtgctg gaattcgccc ttgatctcgc ctggaaacag
      121 agggaaaaaa gaattgtgcg tcgggctaac gcagcggtgt gtgctgagga attacttggg
      181 agatatatct gcaacacaac atttgtgtcc ctgtacagtt ttgtggactg agcgaggaaa
      241 acaacaaata atttaagttg gctgagagct tccgtatttt caaagactgc cacgtgcctt
      301 aggaatactg ttttatcttc atactttgga tgaattgttc gttttttttc ctctccctct
      361 ttttctctct gtatatttat gaccagagca aaatgtaaaa aaggaaaaaa caaaaaatgt
      421 ttgttacttt gaatagtcct aaaaagaaaa aggaaaaaaa aataaggaaa aaaaaatcaa
      481 atcccctcca acggtcgctt tcttgtttaa gggcgaattc tgcagatatc catcacactg
      541 gcggccgctc gagcatgcat ctagagggcc caattcgccc tatagtgagt cgtattacaa
      601 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
      661 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
      721 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca
      781 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
      841 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
      901 caagctctaa atcgggggct cc
//
