LOCUS       sequence-65145-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  TITLE     Macklis lab in situ probes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCRII-Topo Semcap3
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..23
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5..22
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(792..811)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(793..811)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(818..835)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(818..834)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(827..849)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 agctatttag gtgacactat agaatactca agctatgcat caagcttggt accgagctcg
       61 gatccactag taacggccgc cagtgtgctg gaattcgccc ttcctggctc tgttttcagt
      121 ggagggaaaa tacaccctta ctctcttaga aggcaatatt aacaagcagc tttttttcaa
      181 atagcaatgg taatttttta cttgttaacc tttttcataa agtgtttaaa tttccaaaag
      241 atcttttatt aagcatactt tcacagaata atttgtttaa actatattca tataaaagaa
      301 ggttaaacac gctttttttc tgcctaaaac acaaaatgca actgccagta tgtattttta
      361 atgggtccct attttgtaat gtcacttcgc tgaatgtgtt tcatacaagt caccattcat
      421 tcatacagct tatatcagtt ggggtttcaa cacaaaccag ccaaagtctg gttggtgatt
      481 tgacacacac acacatacat acacacacac acacacacac taaccaaact agcagcattt
      541 ctagtcggga tatccatttt taacattttt atggttaagg ttcccaagaa gagtgtcaag
      601 gttttaacag aaagcaaaat ttcctgcagc tttgtggacg cttaaagcat gtttgcaaat
      661 attgccgcct gttggaagaa tttgcatgta cagggaagtc gcggatggag acctgtttgt
      721 ggaagggcga attctgcaga tatccatcac actggcggcc gctcgagcat gcatctagag
      781 ggcccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc
      841 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
      901 ccagctggcg taatagcgaa
//
