LOCUS       sequence-65148-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Subauste MC, Von Herrath M, Benard V, Chamberlain CE, Chuang TH, Chu
            K, Bokoch GM, Hahn KM
  TITLE     Rho family proteins modulate rapid apoptosis induced by cytotoxic T 
            lymphocytes and Fas.
  JOURNAL   J Biol Chem. 2000 Mar 31. 275(13):9725-33.
  PUBMED    10734125
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2000 Mar 31. 275(13):9725-33."
COMMENT     Sequence Label: pcDNA3-EGFP-Cdc42-Q61L
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(9..188,189..191,192..584)
                     /codon_start=1
                     /product="constitutively active mutant of human Rho family 
                     small GTPase Cdc42"
                     /label=Cdc42 (Q61L)
                     /translation="MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVM
                     IGGEPYTLGLFDTAGLEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHH
                     CPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQK
                     GLKNVFDEAILAALEPPEPKKSRRCVLL"
     promoter        complement(612..630)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(613..630)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(650..667)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    656..880
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 aggaattcat gcagacaatt aagtgtgttg ttgtgggcga tggtgctgtt ggtaaaacat
       61 gtctcctgat atcctacaca acaaacaaat ttccatcgga atatgtaccg actgtttttg
      121 acaactatgc agtcacagtt atgattggtg gagaaccata tactcttgga ctttttgata
      181 ctgcagggct agaggattat gacagattac gaccgctgag ttatccacaa acagatgtat
      241 ttctagtctg tttttcagtg gtctctccat cttcatttga aaacgtgaaa gaaaagtggg
      301 tgcctgagat aactcaccac tgtccaaaga ctcctttctt gcttgttggg actcaaattg
      361 atctcagaga tgacccctct actattgaga aacttgccaa gaacaaacag aagcctatca
      421 ctccagagac tgctgaaaag ctggcccgtg acctgaaggc tgtcaagtat gtggagtgtt
      481 ctgcacttac acagaaaggc ctaaagaatg tatttgacga agcaatattg gctgccctgg
      541 agcctccaga accgaagaag agccgcaggt gtgtgctgct atgactcgag catgcatcta
      601 gagggcccta ttctatagtg tcacctaaat gctagagctc gctgatcagc ctcgactgtg
      661 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
      721 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
      781 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
      841 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
      901 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaa
//
