LOCUS       sequence-65151-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lacritin-intein fusion plasmid coding for intact mature lacritin 
            without signal peptide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Wang J, Wang N, Xie J, Walton SC, McKown RL, Raab RW, Ma P, Beck SL,
            Coffman GL, Hussaini IM, Laurie GW
  TITLE     Restricted epithelial proliferation by lacritin via 
            PKCalpha-dependent NFAT and mTOR pathways.
  JOURNAL   J Cell Biol. 2006 Aug 28;174(5):689-700. Epub 2006 Aug 21.
  PUBMED    16923831
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2006-08-28"; volume: "174(5)"; pages: "689-700. Epub 
            2006 Aug 21"
COMMENT     Sequence Label: pLAC
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             486..508
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    523..547
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(547..566)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(548..566)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(701..787)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 atttaattac ctcacgaggt ctgccatctt tacccatgac cttattacca acctcaatgt
       61 tttcaataca ttcaatagac ccatccgcca ttaaaacatt ggtacccttg gcaaagcatg
      121 cccatggttt taatagactg aatttcttca gtaatttttg tgcaaattca cttccatttt
      181 cgatgaattg ttttccacct ggcacgcctc cgtgcattcc ttttcctgct tttgcaaggg
      241 cttgttctgt tagtaagata cttttctcca ctatggattt cagggggttt agttcctgcc
      301 tgcttgaggt gaccttggct gtcccctgaa ctgctgccgc cgaagtctcc tgggctgttg
      361 tggttgtctc tgggggtgaa gctggttctg ctggacctga gatctcttca ttaggcttag
      421 aggtcccagc ttcctgggca ggatcagcac ccgtcgagtc agaggaggca tcttccatat
      481 gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca
      541 caattcccct atagtgagtc gtattaattt cgcgggatcg agatccccgg tttaaaccgg
      601 gaattaattc cgatccccaa ttcctggcag tttatggcgg gcgtcctgcc cgccaccctc
      661 cgggccgttg cttcgcaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag
      721 cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt
      781 ttatttgatg cctggggaat taattcctgg cagtttatgg cgggcgtcct
//
