LOCUS       sequence-65154-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lacritin-intein fusion plasmid coding for truncated lacritin without
            signal peptide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Wang J, Wang N, Xie J, Walton SC, McKown RL, Raab RW, Ma P, Beck SL,
            Coffman GL, Hussaini IM, Laurie GW
  TITLE     Restricted epithelial proliferation by lacritin via 
            PKCalpha-dependent NFAT and mTOR pathways.
  JOURNAL   J Cell Biol. 2006 Aug 28;174(5):689-700. Epub 2006 Aug 21.
  PUBMED    16923831
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2006-08-28"; volume: "174(5)"; pages: "689-700. Epub 
            2006 Aug 21"
COMMENT     Sequence Label: C25-pLAC
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             171..193
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    208..232
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(232..251)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(233..251)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(335..354)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        560..637
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(657..676)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 atttaattac ctcacgaggt ctgccatctt tacccatgac cttattacca acctcaatgt
       61 tttcaataca ttcaatagac ccatccgcca ttaaaacatt ggtacccttg gcaaagcaac
      121 cctccaggga accgattacc agtttacctt cttcgatttt cataagtata tctccttctt
      181 aaagttaaac aaaattattt ctagagggga attgttatcc gctcacaatt cccctatagt
      241 gagtcgtatt aatttcgcgg gatcgagatc tcgatcctct acgccggacg catcgtggcc
      301 ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
      361 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
      421 ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg
      481 gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag
      541 ggagagcgtc gagatcccgg acaccatcga atggcgcaaa acctttcgcg gtatggcatg
      601 atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgttatacga
      661 tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag
      721 ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat
      781 tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac
      841 ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga
      901 tcaactgggt gc
//
