LOCUS       sequence-65157-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Sander JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung JK, Yeh JR
  TITLE     Targeted gene disruption in somatic zebrafish cells using engineered
            TALENs.
  JOURNAL   Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934.
  PUBMED    21822241
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2011-08-5- 5"; volume: "29"; 
            issue: "8"; pages: "697-8"
COMMENT     Sequence Label: TAL3322
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..61)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(77..664)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 ggtgatgatg accggtacgc gtagaatcga gaccgaggag agggttaggg ataggcttac
       61 cttcgaaggg cccttaaaag tttatctcgc cgttattaaa tttccgtctg acttcctcta
      121 aggttaatgt gccggcttta atcatttctc caccaattaa aagctcttct acactaagaa
      181 cagctccatt acaattagtg atatgattta atcgtgtaag ctgagctttg tagtttcctt
      241 taaagtgacc actcacaaat aaaaacttaa attccgttac agaagatgga tagactttcc
      301 accattcatt agggttgata tgtttgtttc gtgtttgatt ttcttcgaca tatcgttgca
      361 tttcatctgc ttggccaatt ggcagattat aacctccgct ataagcttta gtatccacga
      421 tcacaccgta atcaatagga gatccgacag tataaattgc tccgtccggt ttccttgatc
      481 cacccaaatg tttacctcta tatccataaa ctttcataaa aaattccatt accttcattt
      541 caagaattct atcctgagtg gaatttctgg caatttcaat taattcaata tattcatgag
      601 gcacatattt caatttatga cgaagttcag atttcttctc ctccagttca cttttgacta
      661 gttgggatcc cgcgactcga tgggaagttc tctcgggaat ccgccggttg gttcttttga
      721 tcaatgcggg agcatgaggc agaccctttt tgactgcatc gagcgcgggt cgtccaccaa
      781 gacatgccaa cgccaccaga tggtcattcg ttaacgcagc caacgcggga tcgggcctgg
      841 acaattgggc tacgatggac tccaaggctg g
//
