LOCUS       sequence-65158-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Sander JD, Cade L, Khayter C, Reyon D, Peterson RT, Joung JK, Yeh JR
  TITLE     Targeted gene disruption in somatic zebrafish cells using engineered
            TALENs.
  JOURNAL   Nat Biotechnol. 2011 Aug 5;29(8):697-8. doi: 10.1038/nbt.1934.
  PUBMED    21822241
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2011-08-5- 5"; volume: "29"; 
            issue: "8"; pages: "697-8"
COMMENT     Sequence Label: TAL3322
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             80..145
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             152..172
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcaccatgg actacaaaga ccatgacggt gattataaag atcatgacat
      121 cgattacaag gatgacgatg acaagatggc ccccaagaag aagaggaagg tgggcattca
      181 ccgcggggta cctatggtgg acttgaggac actcggttat tcgcaacagc aacaggagaa
      241 aatcaagcct aaggtcagga gcaccgtcgc gcaacaccac gaggcgcttg tggggcatgg
      301 cttcactcat gcgcatattg tcgcgctttc acagcaccct gcggcgcttg ggacggtggc
      361 tgtcaaatac caagatatga ttgcggccct gcccgaagcc acgcacgagg caattgtagg
      421 ggtcggtaaa cagtggtcgg gagcgcgagc acttgaggcg ctgctgactg tggcgggtga
      481 gcttaggggg cctccgctcc agctcgacac cgggcagctg ctgaagatcg cgaagagagg
      541 gggagtaaca gcggtagagg cagtgcacgc ctggcgcaat gcgctcaccg gggccccctt
      601 gaacctgacc ccagaccagg tagtcgcaat cgcgaacaat aatgggggaa agcaagccct
      661 ggaaaccgtg caaaggttgt tgccggtcct ttgtcaagac cacggcctta caccggagca
      721 agtcgtggcc attgcaaata ataacggtgg caaacaggct cttgagacgg ttcagagact
      781 tctcccagtt ctctgtcaag cccacgggct gactcccgat caagttgtag cgattgcgtc
      841 gaacattgga gggaaacaag cattggagac tgtccaacgg ctccttcc
//
