LOCUS       sequence-65159-e         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Wang L, Brugge JS, Janes KA
  TITLE     Intersection of FOXO- and RUNX1-mediated gene expression programs in
            single breast epithelial cells during morphogenesis and tumor 
            progression.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Oct 4;108(40):E803-12. doi: 
            10.1073/pnas.1103423108. Epub 2011 Aug 22.
  PUBMED    21873240
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1103423108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-10-4- 4"; volume: "108"; issue: "40"; pages: "E803-12"
COMMENT     Sequence Label: pLKO.1 shRUNX1 puro
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    129..246
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        295..805
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     724..742
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(827..846)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gctttatata tcttgtggaa aggacgaaac accggcctcg aagacatcgg cagaaactcg
       61 agtttctgcc gatgtcttcg aggtttttaa ttctcgacct cgagacaaat ggcagtattc
      121 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
      181 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
      241 aattttcggg tttattacag ggacagcaga gatccacttt ggccgcggct cgagggggtt
      301 ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg
      361 tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg
      421 cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc
      481 gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc
      541 cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg
      601 accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg
      661 ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc
      721 gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga
      781 ccgaatcacc gacctctctc cccaggggga tccaccggag cttaccatga ccgagtacaa
      841 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
      901 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
      961 ggtcaccgag ctgca
//
