LOCUS       sequence-65161-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Bandyopadhyay P, Xiao H, Coleman HA, Price-Whelan A, Steinman HM
  TITLE     Icm/dot-independent entry of Legionella pneumophila into amoeba and 
            macrophage hosts.
  JOURNAL   Infect Immun. 2004 Aug;72(8):4541-51.
  PUBMED    15271914
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect 
            Immun."; date: "2004-08"; volume: "72(8)"; pages: "4541-51"
COMMENT     Sequence Label: pJN105-Hygro-GFP
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(74..96)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    85..101
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(109..115,116..131,132..137))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     primer_bind     complement(283..299)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(283..299)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(331..350)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(332..350)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(360..377)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(360..376)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(369..391)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttctccttta ctcatatgta tatctccttc ttaaatctag aggatccccg ggtaccgagc
       61 tcgaattctg tttcctgtgt gaaattgtta tccgctcaca attccacaca ttatacgagc
      121 cgatgattaa ttgtcaacag ctcatttcag aatatttgcc agaaccgtta tgatgtcggc
      181 gcgctagctg gaccccagaa ccgttatgat gtcggcgcgc tagcgtggtg cttggctgtt
      241 ttggcggatg agagagagct agcgaattcc tgcagcccgg gggatccact agttctagag
      301 cggccgccac cgcggtggag ctccaattcg ccctatagtg agtcgtatta cgcgcgctca
      361 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
      421 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
      481 ccttcccaac agttgcgcag cctgaatggc gaatggaaat tgtaagcgtt aatattttgt
      541 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgtactgc
      601 gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct
      661 ggttgctacg cctgaataag tgataataag cggatgaatg gcagaaattc gaaagcaaat
      721 tcgacccggt cgtcggttca gggcagggtc gttaaatagc cgcttatgtc tattgctggt
      781 ttaccggttt attgactacc ggaagcagtg tgaccgtgtg cttctcaaat gcctgaggcc
      841 agtttgctca ggctctcccc gtgga
//
