LOCUS       sequence-65179-e         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Pitcher JA, Tesmer JJ, Freeman JL, Capel WD, Stone WC, Lefkowitz RJ
  TITLE     Feedback inhibition of G protein-coupled receptor kinase 2 (GRK2) 
            activity by extracellular signal-regulated kinases.
  JOURNAL   J Biol Chem. 1999 Dec 3. 274(49):34531-4.
  PUBMED    10574913
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1999 Dec 3. 274(49):34531-4."
COMMENT     Sequence Label: pcDNA3 GRK2 S670D
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..48
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        29..47
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      121..130
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
       61 ggtaccgagc tcggatccac tagtaacggc cgccagtgtg ctggaattcg cgtcgacgcc
      121 gccaccatgg cggacctgga ggcggtgctg gccgacgtga gctacctgat ggccatggag
      181 aagagcaagg ccacgccggc ggcgcgcgcc agcaagaaga tcctgctgcc cgagcccagc
      241 atccgcagcg tcatgcagaa gtacctggag gaccggggcg aggtgacttt tgagaagatc
      301 ttctcccaga agctggggta cctgcttttc cgagacttct gcctgaagca cctggaggag
      361 gccaagccct tggtagagtt ctacgaggag atcaagaaat acgagaagct ggagacagag
      421 gaggagcgcc tggtctgcag ccgagagatc ttcgacacgt acatcatgaa ggagctgctg
      481 gcctgctcac atcctttctc gaagagcgcc attgagcacg tccagggcca tctggtgaag
      541 aagcaggtgc ctccggatct cttccagcca tatattgaag aaatttgcca gaacctccga
      601 ggagacgtgt tccagaaatt catcgagagc gataaattca cacgattttg ccagtggaag
      661 aatgtagagc tcaacatcca cctgaccatg aacgacttca gtgtgcaccg catcatcggg
      721 cgaggcggct tcggtgaggt ctacggctgc cggaaggccg acacgggcaa
//
