LOCUS       sequence-65206-e        3424 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3424)
  TITLE     Craig lab unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: p401F/GgVcl 1-1066 (N term FLAG tag)
FEATURES             Location/Qualifiers
     source          1..3424
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        48..66
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     48..65
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     79..98
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      143..152
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             152..175
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
       61 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      121 acctcggttc tatcgatcag ccgccaccat ggactacaag gacgacgatg acaagggatc
      181 caaagaattc gcccttcata tgatgcccgt cttccacacg cgcaccatcg agagcatctt
      241 ggagcccgtg gctcagcaga tctcccacct ggtcatcatg cacgaggagg gggaggtaga
      301 cggcaaggcc atcccggacc tcaccgcccc cgtgtcggcc gtgcaggccg ctgtcagcaa
      361 cctggtgcgg gttggaaaag aaactgtgca gacaacagaa gaccagatct tgaaaaggga
      421 tatgccacca gcattcatca aagtagagaa tgcctgcacc aagctcgttc gagcagccca
      481 gatgctgcaa gcagatcctt attcagtacc agctcgtgac tacctaattg atggatcaag
      541 aggcatcctt tctggaacat cagacttact tctgacattt gatgaagcgg aggtccgtaa
      601 aatcatccgt gtctgcaaag gaatattgga atatctgact gtggcagaag tagtagagac
      661 tatggaggat ttggtgacat atacaaagaa tctagggcca ggaatgacaa agatggcgaa
      721 aatgatcgat gagagacaac aggaattaac tcatcaggaa catagggtta tgctggtgaa
      781 ctccatgaat actgtgaagg agctattgcc tgtacttatt tcagctatga agatctttgt
      841 aaccacaaaa aacactaaaa gccagggaat agaagaggcc ttgaaaaatc gcaatttcac
      901 agtagagaaa atgagtgctg agataaatga aataatccgt gtattacaac tcacttcctg
      961 ggatgaagat gcctgggcca gcaaggacac tgaagccatg aaaagagctt tagccctaat
     1021 agattcaaag atgaaccagg caaaaggctg gctgagagat ccaaacgcac ctccagggga
     1081 tgctggtgag caagcaataa ggcagatcct tgatgaagct ggaaaagcag gagaattgtg
     1141 tgcaggcaaa gaacgcaggg agattctggg aacatgcaaa actttgggcc aaatgactga
     1201 tcaacttgct gatctccgag ctagaggaca gggtgctaca ccgatggcaa tgcagaaggc
     1261 acagcaggtg tcacaaggcc tggatttgct cactgcaaaa gtggagaatg cagcccggaa
     1321 attggaggcc atgacaaact ctaagcaggc tattgcaaag aagattgatg ctgctcagaa
     1381 ctggcttgcg gatcccaacg gcggaagtga aggagaagaa cacattcgag gaattatgtc
     1441 tgaagcaagg aaagttgcag aattgtgtga ggagcctaaa gaaagagatg atatccttcg
     1501 ctccttgggg gaaatctctg ctctgacagc taagctgtca gatctgcgac gacatgggaa
     1561 aggcgactct cctgaggccc gtgcattggc caagcaaata gctacatcac ttcagaactt
     1621 acagtccaaa acaaacagag ctgtagcaaa tactaggcca gttaaagctg ctgtccattt
     1681 ggagggcaag attgagcaag cccagaggtg gatagataat cctacagttg atgatcgggg
     1741 agtaggccag gcagcaattc ggggtttggt tgcagaaggt cgtcgtctag ccaatgtcat
     1801 gatgggacct tatcgtcagg acctgcttgc caaatgtgac cgtgtagacc agctggctgc
     1861 tcagcttgct gaccttgcag caagagggga aggagagtct cctcaggcta gggcaattgc
     1921 tgctcagctt caggactccc tgaaggatct caaagcacgg atgcaagaag caatgaccca
     1981 ggaggtgtct gatgttttca gtgacactac aacccctatt aagttgttag cagtagcagc
     2041 cactgctcct tctgatactc ccaatagaga agaggtgttt gaagaaagag cagcaaattt
     2101 tgaaaaccat gctgctagac tgggagcaac agcagaaaaa gcagctgcag ttggaactgc
     2161 taataaaact actgtggaag gcattcaggc aacagtcaaa tctgcaaggg agcttacacc
     2221 acaggtagta tcggctgctc gaatcctcct gagaaatcct ggaaatcaag ctgcttatga
     2281 gcattttgag acaatgaaaa accaatggat tgataatgta gaaaagatga cagggctggt
     2341 ggatgaggcc atcgatacca agtctctgtt ggatgcatca gaagaggcta ttaagaagga
     2401 tcttgataaa tgcaaagttg caatggccaa tatgcaacct cagatgctgg tagctggagc
     2461 caccagcatt gctagacgag cgaaccgcat cctgcttgtg gcaaaacggg aggttgaaaa
     2521 ttcagaagac cctaaattca gggaggctgt taaagcagct tctgatgagc tgagcaaaac
     2581 catatcaccg atggtaatgg atgctaaagc tgtagcagga aatatctctg atcctggttt
     2641 gcagaagagt ttcttggatt ctggatacag gattctggga gctgtggcca aagtcagaga
     2701 agcctttcag cctcaggagc cagactttcc ccctcctcct cctgacctag agcatctcca
     2761 tctgactgat gagcttgctc ctccaaaacc accacttcca gaaggtgagg ttcccccacc
     2821 cagaccacca ccccctgaag aaaaagatga ggagttccca gagcagaaag caggagaagc
     2881 tattaatcag cccatgatga tggctgctag gcagttgcat gacgaggctc ggaaatggtc
     2941 tagcaagggt aacgacatca ttgctgctgc taaacgaatg gcgctgctca tggctgagat
     3001 gtcacgcctg gtgcgaggag gcagcggaaa caagcgtgcc ctcatccagt gtgcaaaaga
     3061 tattgctaag gcatcggatg aagtcactcg attggccaaa gaggtggcca agcaatgtac
     3121 tgataaacgc attagaacaa acctcttaca ggtctgtgag cgaatcccaa ccatcagcac
     3181 gcagctcaaa attctttcca cagtgaaagc taccatgctg ggcaggacta acatcagcga
     3241 tgaagaatca gaacaggcaa ctgagatgtt ggttcataac gcccagaacc tcatgcagtc
     3301 tgtgaaggaa actgtgagag aagctgaagc agcatccatt aagataagaa cagatgccgg
     3361 attcactctg cgctgggtca gaaagacccc atggtatcag taactcgagg gggggcccgg
     3421 tacc
//
