LOCUS       sequence-65213-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Zhong H, Sia GM, Sato TR, Gray NW, Mao T, Khuchua Z, Huganir RL, 
            Svoboda K
  TITLE     Subcellular dynamics of type II PKA in neurons.
  JOURNAL   Neuron. 2009 May 14;62(3):363-74. doi: 10.1016/j.neuron.2009.03.013.
  PUBMED    19447092
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2009.03"; journalName: "Neuron"; date: 
            "2009-05-14- 14"; volume: "62"; issue: "3"; pages: "363-74"
COMMENT     Sequence Label: pCDNA3-mouse PKA-RIalpha-mEGFP
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             111..128
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac
       61 gactcactat agggagaccc aagcttgcgg ccgccaccat gcggggttct catcatcatc
      121 atcatcatgg tatggctagc atggcgtctg gcagtatggc aaccagtgag gaagagcgga
      181 gtctccggga atgcgagctc tatgtgcaga agcacaatat ccaggccctg ctgaaggact
      241 ccatcgtgca gctgtgcact acgcggcccg agaggcccat ggcattcctt cgggaatact
      301 ttgagaggtt ggagaaggag gaggcaagac agattcagtg tctacagaaa accggcatcc
      361 gtactgactc gagggaggac gagatctctc ctccaccccc caatccagtg gtgaagggcc
      421 gacggcgccg aggtgctatc agtgctgaag tttacactga ggaggatgct gcctcctacg
      481 ttagaaaggt tattccaaaa gattataaga caatggctgc tttagccaag gccatcgaaa
      541 agaatgtgct gttttcacac cttgatgata acgagagaag tgacattttt gatgctatgt
      601 ttccagtctc ctttattgct ggagagacgg ttattcagca aggtgatgaa ggggataact
      661 tctatgtgat tgatcaagga gaaatggatg tctatgtcaa taatgaatgg gcaaccagtg
      721 ttggggaagg agggagcttt ggagagctgg ctttgattta tggaacaccc agagcagcca
      781 ctgtcaaagc aaagacaaac gtgaaactgt ggggcatcga ccgagacagc tacc
//
