LOCUS       sequence-65215-e         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Zhong H, Sia GM, Sato TR, Gray NW, Mao T, Khuchua Z, Huganir RL, 
            Svoboda K
  TITLE     Subcellular dynamics of type II PKA in neurons.
  JOURNAL   Neuron. 2009 May 14;62(3):363-74. doi: 10.1016/j.neuron.2009.03.013.
  PUBMED    19447092
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2009.03"; journalName: "Neuron"; date: 
            "2009-05-14- 14"; volume: "62"; issue: "3"; pages: "363-74"
COMMENT     Sequence Label: pcDNA3-mouse PKA-RIbeta-mEGFP
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             110..127
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttgcggc cgccaccatg cggggttctc atcatcatca
      121 tcatcatggt atggctagca tggcctcccc atcatgcttc cactcggagg atgaggactc
      181 tctgaaagga tgcgagatgt acgtgcagaa acatggcatc cagcaggtgc tcaaagaatg
      241 cattgtgcac ctctgtgtcg ccaagccgga ccggccactg cgattcctcc gggagcactt
      301 tgagaagttg gagaaggagg aaaacaggca gatcctggct cggcagaagt caaactccca
      361 gtgtgattcc cacgatgagg agatctcccc aacacctcca aaccccgtgg tcaaggcgcg
      421 tcgccggcgg ggcggtgtga gtgctgaagt ctacactgaa gaagatgctg tctcctacgt
      481 gaggaaggtc attcccaagg actataagac catgaccgcg ctggccaagg ccatttctaa
      541 gaacgtgctc ttttctcacc tggacgacaa cgagagaagt gacatatttg acgccatgtt
      601 tcctgtcact cacatcggtg gggaaacagt catacagcaa gggaatgaag gagataattt
      661 ctatgtgatt gaccaaggag aagtagatgt atatgtgaac ggggaatggg tgaccaacat
      721 cagtgagggg ggaagcttcg gggagctggc tctcatctac ggcaccccca gagcggctac
      781 cgtgaaggcc aagacggacc tcaagctctg gggtatcgac cgtgacagct acaggcgcat
      841 cctcatggga agcacactga ggaaacgcaa gatgtatgag gagttcctca gcaaagtctc
      901 catcctagaa tccctggaga agtgggaacg cctgactgta
//
