LOCUS       sequence-65224-e         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Zhong H, Sia GM, Sato TR, Gray NW, Mao T, Khuchua Z, Huganir RL, 
            Svoboda K
  TITLE     Subcellular dynamics of type II PKA in neurons.
  JOURNAL   Neuron. 2009 May 14;62(3):363-74. doi: 10.1016/j.neuron.2009.03.013.
  PUBMED    19447092
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2009.03"; journalName: "Neuron"; date: 
            "2009-05-14- 14"; volume: "62"; issue: "3"; pages: "363-74"
COMMENT     Sequence Label: pCDNA3-mouse PKA-RIIalpha-mEGFP
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             110..127
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttgcggc cgccaccatg cggggttctc atcatcatca
      121 tcatcatggt atggctagca tgagccacat ccagatcccc gcggggctca cggagctgct
      181 gcagggctac accgtggagg tgcttcggca gcagccgccc gacctcgtcg acttcgcggt
      241 ggagtacttc acacgcctgc gcgaggcccg ccgccaggaa tcagacacgt tcatcgtctc
      301 cccgacgacc tttcacacgc aggagtccag cgcagtcccc gtcatcgagg aggacgggga
      361 gagtgactcg gactcggaag atgccgatct ggaagttccg gttcctagca aatttactag
      421 acgagtatca gtctgtgcag aaacgtttaa ccctgatgaa gaagaggagg ataacgatcc
      481 aagggtggtt catcccaaaa ctgatgagca gagatgccgg cttcaggaag cttgtaaaga
      541 tattcttctt ttcaaaaacc ttgatcagga acagctttct caagttctgg atgccatgtt
      601 tgaaaagatt gtcaaaactg acgagcatgt cattgaccaa ggcgacgacg gggacaactt
      661 ttatgtcata gaacggggaa cctatgacat tttagtaacg aaagataatc aaacacgttc
      721 tgttggtcag tatgacaacc gtggcagttt tggagaacta gctctgatgt acaatacccc
      781 gagagctgct accatcatcg ccacctcaga aggctccctt tggggattgg accgggtgac
      841 ttttagaaga atcatagtga aaaacaatgc aaagaagagg aagatgtttg aatcatttat
      901 tgagtctgtt ccactcttta agtcactaga gatgtcagaa cgaatgaaga ttgtggatgt
      961 gatcggggaa aagatctata aggacggaga gcgaataat
//
