LOCUS       sequence-65246-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Shin J, Noireaux V
  TITLE     Efficient cell-free expression with the endogenous E. Coli RNA 
            polymerase and sigma factor 70.
  JOURNAL   J Biol Eng. 2010 Jun 24;4:8.
  PUBMED    20576148
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2010-06-24"; volume: "4"; pages: "8"
COMMENT     Sequence Label: pBADmod1-linker-deGFP
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..51)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(76..97)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     425..444
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     684..705
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(769..934)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(827..846)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 cgccaaaaca gccaagctgg agaccttaat tcagtggtgg tggtggtggt gctcgagtta
       61 gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc
      121 tttgctcagg gcggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc
      181 gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt
      241 gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac
      301 gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa
      361 gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc
      421 gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc
      481 gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca
      541 ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt
      601 ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga
      661 cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gaacaacgcc
      721 agtgaaaagc tccatggtat atctccttct taaagttaaa aaacgggtat ggagaaacag
      781 tagagagttg cgataaaaag cgtcaggtag gatccgctaa tcttatggat aaaaatgcta
      841 tggcatagca aagtgtgacg ccgtgcaaat aatcaatgtg gacttttctg ccgtgattat
      901 agacactttt gttacgcgtt tttgtcatgg ctttg
//
