LOCUS       sequence-65248-e        1046 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Shin J, Noireaux V
  TITLE     Efficient cell-free expression with the endogenous E. Coli RNA 
            polymerase and sigma factor 70.
  JOURNAL   J Biol Eng. 2010 Jun 24;4:8.
  PUBMED    20576148
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2010-06-24"; volume: "4"; pages: "8"
COMMENT     Sequence Label: pBEST-p15A-PLtetO1-deGFP-T500
FEATURES             Location/Qualifiers
     source          1..1046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             17..39
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    63..81
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    88..106
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(377..395)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        463..567
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(786..805)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaagctccat ggtatatctc cttcttaaag ttaaacaaaa ttattgctag ctgtgctcag
       61 tatctctatc actgataggg atgtcaatct ctatcactga tagggagcat gcccagcgga
      121 acagcacaca catgtcgcgg tggttacgtc cgtcacgttc acgcatcagg cggatatcgt
      181 tagcccaccc agcaaaattc ggttttctgg ctgatggtgc gatagtcttc accatgtcaa
      241 acatccactc tgcggcggtc aggtcttctg ctgtccccca cttgctggcg ctctgaattg
      301 cagcatccgg tttcaccaca gaaaggtcgt tttctggctg gtcagaggat tcgccagaat
      361 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
      421 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
      481 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
      541 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
      601 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
      661 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
      721 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
      781 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
      841 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
      901 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
      961 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     1021 aacatggggg atcatgtaac tcgcct
//
