LOCUS       sequence-65249-e        1063 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Shin J, Noireaux V
  TITLE     Efficient cell-free expression with the endogenous E. Coli RNA 
            polymerase and sigma factor 70.
  JOURNAL   J Biol Eng. 2010 Jun 24;4:8.
  PUBMED    20576148
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2010-06-24"; volume: "4"; pages: "8"
COMMENT     Sequence Label: pBEST-p15A-PLlacO1-lacI-T500
FEATURES             Location/Qualifiers
     source          1..1063
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    50..66
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(363..381)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        449..553
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(772..791)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ggatccttct taaagttaaa caaaattatt gctagctgtg ctcagtatct tgttatccgc
       61 tcacaatgtc aatgttatcc gctcacattt atgcatgccc agcggaacag cacacacatg
      121 tcgcggtggt tacgtccgtc acgttcacgc atcaggcgga tatcgttagc ccacccagca
      181 aaattcggtt ttctggctga tggtgcgata gtcttcacca tgtcaaacat ccactctgcg
      241 gcggtcaggt cttctgctgt cccccacttg ctggcgctct gaattgcagc atccggtttc
      301 accacagaaa ggtcgttttc tggctggtca gaggattcgc cagaattctt gaagacgaaa
      361 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
      421 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
      481 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
      541 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
      601 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
      661 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
      721 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
      781 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
      841 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
      901 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
      961 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     1021 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gcc
//
