LOCUS       sequence-65250-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Shin J, Noireaux V
  TITLE     Efficient cell-free expression with the endogenous E. Coli RNA 
            polymerase and sigma factor 70.
  JOURNAL   J Biol Eng. 2010 Jun 24;4:8.
  PUBMED    20576148
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2010-06-24"; volume: "4"; pages: "8"
COMMENT     Sequence Label: pBEST-p15A-PLlacO1-deGFP-T500
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             17..39
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    65..81
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(378..396)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        464..568
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(787..806)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaagctccat ggtatatctc cttcttaaag ttaaacaaaa ttattgctag ctgtgctcag
       61 tatcttgtta tccgctcaca atgtcaatgt tatccgctca catttatgca tgcccagcgg
      121 aacagcacac acatgtcgcg gtggttacgt ccgtcacgtt cacgcatcag gcggatatcg
      181 ttagcccacc cagcaaaatt cggttttctg gctgatggtg cgatagtctt caccatgtca
      241 aacatccact ctgcggcggt caggtcttct gctgtccccc acttgctggc gctctgaatt
      301 gcagcatccg gtttcaccac agaaaggtcg ttttctggct ggtcagagga ttcgccagaa
      361 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
      421 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      481 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      541 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      601 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      661 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      721 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      781 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
      841 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcaca
//
