LOCUS       sequence-65251-e         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Shin J, Noireaux V
  TITLE     Efficient cell-free expression with the endogenous E. Coli RNA 
            polymerase and sigma factor 70.
  JOURNAL   J Biol Eng. 2010 Jun 24;4:8.
  PUBMED    20576148
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2010-06-24"; volume: "4"; pages: "8"
COMMENT     Sequence Label: pBEST-melR-deGFP-T500
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 aagctccatg gtatatctcc ttcttaaagt taaacaaaat tattgctagc tgaataactt
       61 catcatggca ggcgaatctg cagaaaacct ccggcttcca ggttgagcat tcccataaac
      121 tcagatcctg cgtgaagcag cagtaaatct gagtttatgg gaataaatta cctgaaaagc
      181 agagttttcg gcctcatgat actcggagag tcccctcgcc cctctgggaa gagggtgaaa
      241 accgtgctcc cactcgcagt catcctccct cactcctgcc ataattctga tattccagga
      301 aagagagcca tccatgaata cagatacgtt tatgtgcagc agcgacgaaa aacagacccg
      361 cagcccgctg tcgctgtatt cggaatacca gcggatggag attgagtttc gcgcaccgca
      421 tatcatgccc accagccact ggcatggtca ggtcgaagtg aatgtgcctt tcgatggcga
      481 tgtggaatac ctgatcaaca atgaaaaagt gaatatcaat cagggtcata tcacgctgtt
      541 ctgggcctgt acaccgcacc aactaacaga taccggaacc tgtcagagca tggcgatttt
      601 taatctgccg atgcatctgt ttctctcctg gccgctggat aaagacctga ttaaccacgt
      661 cactcacggc atggtgatca aatcactggc cacacagcaa cttagcccgt ttgaagtgcg
      721 ccgctggcag caggaattaa acagtccgaa cgagcaaatt cgccagctcg ccattgatga
      781 aattggcctg atgctcaagc gatttagcct ctctggctgg gaaccgattc tggtcaataa
      841 aacctcgcgc acacacaaaa acagcgtctc gcgccatgcg caattttatg ttagccagat
      901 gctgggcttt attgccgaaa actatgatca ggcgctgacc atcaacgatg tggctgagca
      961 cgtcaaactt aacgccaact atgcaatggg
//
