LOCUS       sequence-65279-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Sullivan EK, Weirich CS, Guyon JR, Sif S, Kingston RE
  TITLE     Transcriptional activation domains of human heat shock factor 1 
            recruit human SWI/SNF.
  JOURNAL   Mol Cell Biol 2001 Sep;21(17):5826-37.
  PUBMED    11486022
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol"; date: "2001-09"; volume: "21(17)"; pages: "5826-37"
COMMENT     Sequence Label: pBABE Gal4 (1-94) HSF ABC Flag wt
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(43..66)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(67..438)
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
ORIGIN
        1 attccttttt tttagagcct gtctgtttat agatcttcgc tacttgtcat cgtcgtcctt
       61 gtagtcggag acagtggggt ccttggcttt gggaggctcc gagcctgtca gcagggagat
      121 ggtggggtcc tcggcgaagc cgtccccttc ggagaagtag gagccctctc ccagctcaaa
      181 cagcaccggc aggtcgttgc tcccggtgtc cacggagccg gggtccagca ggaacagcgg
      241 ctgcgctgtg tagtgcacca gctgcttccc tgaatccggg ctgctgttct ctgcctcggg
      301 aggcctgggg ggctcctggg gagacaggag ctcttggata ctggccaggc tgctgtcaag
      361 gtcaggcagg ctcatgtcgg gcacggtcac cgaggggctg aacaggtcca gcagggcact
      421 ggtgtccacg ctgaagccgt ggctgctcag catggtctgc aggttatcca ggttggagtc
      481 catagcatcc aagtggtcac tgagctcatt cttgtccagg caggctacgc tgaggcactt
      541 ttcaggggtg gaggtgggcg ggggggaggg aggccggccc tcggtgtccg tgtggcccct
      601 ggcgtccgtg agggctgtga cggaggcggg ggcaggttca ctctcccgca ggatggagtc
      661 aatgagggcg gtcggggaca agagggtgtc cacggaagat gggcgcccgg gactcgcctc
      721 ctctacccgg gggctctgag gcgggctggg gggctcctcc ttgacacgca ccagggggct
      781 gctggatagg ggcctctcgt ctatgctccc gccgggggag gccatggggc tggcaggagc
      841 cagctcggtg atgtcggaga tgatgggtcc agagctggcc acagcatcag gggcgta
//
