LOCUS       sequence-65297-e        2628 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Bar-Peled L, Chantranupong L, Cherniack AD, Chen WW, Ottina KA, 
            Grabiner BC, Spear ED, Carter SL, Meyerson M, Sabatini DM
  TITLE     A Tumor suppressor complex with GAP activity for the Rag GTPases 
            that signal amino acid sufficiency to mTORC1.
  JOURNAL   Science. 2013 May 31;340(6136):1100-6. doi: 10.1126/science.1232044.
  PUBMED    23723238
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2013-05-31- 31"; volume: "340"; 
            issue: "6136"; pages: "1100-6"
COMMENT     Sequence Label: C terminal Flag Mios pRK5
FEATURES             Location/Qualifiers
     source          1..2628
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1019..1030)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 atgagcggta ccaaacctga tattttatgg gcaccacacc atgttgatag atttgttgtg
       61 tgtgactcag aactaagtct ttatcatgtg gaatctactg tgaattcaga actcaaagct
      121 ggatctttac gtttatctga agactctgca gctacattac tgtcaataaa ttcagataca
      181 ccctatatga aatgtgttgc ctggtatctt aattatgatc ctgaatgtct gctggcagtt
      241 ggacaagcaa atggtcgagt tgtacttaca agccttggtc aagatcataa ctcaaagttc
      301 aaagatttga taggaaaaga gtttgttcca aaacatgcac gacaatgtaa tacccttgcc
      361 tggaatccac tggatagtaa ctggctagct gctggtttag ataagcacag agctgacttt
      421 tcagtgctaa tatgggatat ctgcagcaaa tatactcctg atatagttcc catggaaaaa
      481 gtgaaacttt cagcaggtga aactgaaaca acattattag taacaaaacc actttatgag
      541 ttaggacaga atgatgcttg tctgtctctt tgttggcttc cacgagacca gaaacttctc
      601 cttgctggta tgcatcgtaa cctagctata tttgatcttc ggaatacaag ccaaaagatg
      661 ttcgtaaata caaaagctgt tcagggtgtg acggtagacc catatttcca cgatcgtgtt
      721 gcttccttct atgaaggtca ggttgcaata tgggatctta gaaaatttga gaagccagtt
      781 ttgacattga ctgagcaacc aaaaccctta acaaaagtag catggtgtcc cactaggact
      841 ggtctacttg ccactttaac aagggatagt aatattatta gattgtatga tatgcagcat
      901 acacccactc ccattgggga tgaaactgaa cccacaataa ttgaaagaag tgtgcaacct
      961 tgtgacaatt acattgcttc ctttgcgtgg catccaacaa gtcaaaatcg aatgatagtt
     1021 gtaactccca accgaacaat gtcagacttc actgtttttg aaaggatatc tcttgcctgg
     1081 agcccaatta catctttaat gtgggcttgt ggtcgtcatt tatatgaatg tacggaagaa
     1141 gaaaatgata attctttaga aaaagatata gcaacgaaga tgcgtcttcg ggctttatca
     1201 aggtatggac ttgatacaga gcaggtgtgg aggaaccaca ttttagctgg aaatgaagat
     1261 ccacagctca agtcactctg gtatactctg cactttatga agcaatacac agaagatatg
     1321 gatcagaaat ctccaggcaa caaaggatca ttggtttatg caggaattaa atcaattgta
     1381 aagtcatcgt tgggaatggt ggaaagcagc agacataatt ggagtgggtt ggataagcaa
     1441 agtgatattc aaaatttaaa tgaagagaga atcttagctt tacagctttg tgggtggata
     1501 aagaaaggaa cggatgtaga cgtggggcca tttttgaact cccttgtaca agaaggggaa
     1561 tgggaaagag ctgctgctgt ggcattgttc aacttggata ttcgccgagc aatccaaatc
     1621 ctgaatgaag gggcatcttc tgaaaaagga gatctgaatc tcaatgtggt agcaatggct
     1681 ttatcgggtt atacggatga gaagaactcc ctttggagag aaatgtgtag cacactgcga
     1741 ttacagctaa ataacccgta tttgtgtgtc atgtttgcat ttctgacaag tgaaacagga
     1801 tcttacgatg gagttttgta tgaaaacaaa gttgcagtac gtgacagagt ggcatttgct
     1861 tgtaaattcc ttagtgatac tcagttaaat agatacatcg aaaagttgac caatgaaatg
     1921 aaagaggctg gaaatttgga aggaattttg cttacaggcc ttactaaaga tggagtggac
     1981 ttaatggaga gttatgttga tagaactgga gatgttcaaa cagcaagtta ctgtatgtta
     2041 cagggttcac ctttagatgt tcttaaagat gaaagggttc agtactggat tgagaattat
     2101 agaaatttat tagatgcctg gaggttttgg cataaacgag ctgaatttga tattcacagg
     2161 agtaagttgg atcccagttc caagccttta gcacaagttt ttgtgagttg caatttctgt
     2221 ggcaagtcaa tctcctacag ctgttcagct gtgcctcatc agggcagagg ttttagtcag
     2281 tatggtgtga gtggctcacc aacgaaatct aaagtcacaa gttgtcctgg ctgtcgaaaa
     2341 ccacttcctc gatgtgcgct ttgtctcatt aatatgggaa caccagtttc tagctgtcct
     2401 ggaggaacca aatcagatga aaaagtggac ttgagcaagg acaaaaaatt agcccaattt
     2461 aacaactggt ttacatggtg tcataattgc aggcacggtg gacatgctgg acatatgctt
     2521 agttggttca gggaccatgc agagtgccct gtgtcggcat gcacgtgtaa atgtatgcag
     2581 ttggatacaa cagggaatct ggtacctgca gagactgtcc agccataa
//
