LOCUS       sequence-65327-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis.
  JOURNAL   Cell. 2010 Apr 30. 141(3):536-48.
  PUBMED    20434990
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 30. 141(3):536-48."
COMMENT     Sequence Label: pQUAST
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(270..290)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          420..485
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     primer_bind     complement(678..699)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
       61 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctctagt caaggcacta
      121 tacatcaaat atttttccat aattttcttg tatagcagtg cagctttttc ctttgtggtg
      181 taaatagcaa agcaagcaag agttctatta ctaaacacag catgactcaa aaaacttagc
      241 aattctgaag gaaagtcctt ggggtcttct acctttctct tcttttttgg aggagtagaa
      301 tgttgagagt cagcagtagc ctcatcatca ctagatggca tttcttctga gcaaaacagg
      361 ttttcctcat taaaggcatt ccaccactgc tcccattcat cagttccata ggttggaatc
      421 taaaatacac aaacaattag aatcagtagt ttaacacatt atacacttaa aaattttata
      481 tttaccttag agctttaaat ctctgtaggt agtttgtcca attatgtcac accacagaag
      541 taaggttcct tcacaaagat cctctagagg taccctcgag ccgcggccgc agatctgtta
      601 acgaattgat cccccgatct gttaacgaat tcccaattcc ctattcagag ttctcttctt
      661 gtattcaata attacttctt ggcagatttc agtagttgca gttgatttac ttggttgctg
      721 gttactttta attgattcac tttaacttgc actttactgc agattgttta gcttgtt
//
