LOCUS       sequence-65337-e         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: CBR3
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..83
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        64..82
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    83..107
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             122..144
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             155..172
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             197..217
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
       61 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
      121 ttttgtttaa ctttaagaag gagatataca tatgcaccat catcatcatc attcttctgg
      181 tgtagatctg ggtaccgaga acctgtactt ccaatccatg tcgtcctgca gccgcgtggc
      241 gctggtgacc ggggccaaca ggggcatcgg cttggccatc gcgcgcgaac tgtgccgaca
      301 gttctctggg gatgtggtgc tcaccgcgcg ggacgtggcg cggggccagg cggccgtgca
      361 gcagctgcag gcggagggcc tgagcccgcg cttccaccaa ctggacatcg acgacttgca
      421 gagcatccgc gccctgcgcg acttcctgcg caaggagtac ggggggctca atgtactggt
      481 caacaacgcg gccgtcgcct tcaagagtga tgatccaatg ccctttgaca ttaaagctga
      541 gatgacactg aagacaaatt tttttgccac tagaaacatg tgcaacgagt tactgccgat
      601 aatgaaacct catgggagag tggtgaatat cagtagtttg cagtgtttaa gggcttttga
      661 aaactgcagt gaagatctgc aggaaaggtt ccacagtgag acactcacag aaggagacct
      721 ggtggatctc atgaaaaagt ttgtggagga cacaaaaaat gaggtgcatg agagggaagg
      781 ctggcccaac tcaccttatg gggtgtccaa gttgggggtc acagtcttat cgaggatcct
      841 ggccaggcgt ctggatgaga agaggaaagc tgacaggatt ctggtgaatg cgtgctgccc
      901 aggaccagtg aagacagaca tggatgggaa agacagcatc aggactgtgg aggagggggc
      961 tgagacccct gtctacttgg cc
//
