LOCUS       sequence-65346-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral eGFP expression vector, CMV promoter, Puro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti CMV GFP Puro (658-5)
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..26
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    75..663
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             181..192
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     590..610
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(724..745)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cccagcgggg ctgctaaagc gcatgctcca gactgccttg ggaaaagcgc ctcccctacc
       61 cggtagaatt ccaggcgggg aggcggccca aagggagatc cgactcgtct gagggcgaag
      121 gcgaagacgc ggaagaggcc gcagagccgg cagcaggccg cgggaaggaa ggtccgctgg
      181 attgagggcc gaagggacgt agcagaagga cgtcccgcgc agaatccagg tggcaacaca
      241 ggcgagcagc catggaaagg acgtcagctt ccccgacaac accacggaat tgtcagtgcc
      301 caacagccga gcccctgtcc agcagcgggc aaggcaggcg gcgatgagtt ccgccgtggc
      361 aatagggagg gggaaagcga aagtcccgga aaggagctga caggtggtgg caatgcccca
      421 accagtgggg gttgcgtcag caaacacagt gcacaccacg ccacgttgcc tgacaacggg
      481 ccacaactcc tcataaagag acagcaacca ggatttatac aaggaggaga aaatgaaagc
      541 catacgggaa gcaatagcat gatacaaagg cattaaagca gcgtatccac atagcgtaaa
      601 aggagcaaca tagttaagaa taccagtcaa tctttcacaa attttgtaat ccagaggttg
      661 attgtcgacg cggccgcttt acttgtacag ctcgtccatg ccgagagtga tcccggcggc
      721 ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc
      781 ggactgggtg ctcaggtagt ggttgtcggg
//
