LOCUS       sequence-65347-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral eGFP expression vector, CMV promoter, Puro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti CMV GFP Puro (658-5)
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     15..35
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        17..35
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        63..289
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             290..470
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    517..642
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
       61 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
      121 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      181 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      241 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      301 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      361 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      421 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      481 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      541 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      601 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      661 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      721 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      781 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      841 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatc
//
