LOCUS       sequence-65352-e        1449 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Fairhead M, Krndija D, Lowe ED, Howarth M
  TITLE     Plug-And-Play Pairing Via Defined Divalent Streptavidins.
  JOURNAL   J Mol Biol. 2013 Sep 19. pii: S0022-2836(13)00600-1. doi: 
            10.1016/j.jmb.2013.09.016.
  PUBMED    24056174
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2013 Sep 19. pii: S0022-2836(13)00600-1. doi: 
            10.1016/j.jmb.2013.09.016."
COMMENT     Sequence Label: pET21-Streptavidin-Glutamate_Tag
FEATURES             Location/Qualifiers
     source          1..1449
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..51)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             488..510
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    525..549
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(549..568)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(550..568)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(652..671)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        877..954
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(974..993)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     gap             1437..1448
                     /estimated_length=12
ORIGIN
        1 cnnnntttcn nnnnngttag cagccggatc tcagtggtgg tggtggtggt gctcgagtgc
       61 ggccgcaagc ttttattact cttcctcttc ctcttcggaa gcagcggacg gtttaacttt
      121 ggtgaaggtg tcgtgaccaa ccagggtgga tttccacgcg ttagcttcgg tggtgccgga
      181 ggtcaacaac cactgggtgt tgatacgagc ttcagcacca ccaacgtact ggccagacca
      241 ggtggtagcg gagtgagcgt tacggtagtt gtttttccaa gcaacggtcc aacccagagc
      301 ggttccggaa ccgtcggtag ccggagcgga gtcgtaacga ccggtcagaa cgtatctaga
      361 ttcagcgtta ccaacagcgg attcgtaggt accggtcaga gcaccgtcag caccagcggt
      421 aacgatgaag gtggatccca gctggttgta ccaggtgccg gtgataccag cttcagccat
      481 atgtatatct ccttcttaaa gttaaacaaa attatttcta gaggggaatt gttatccgct
      541 cacaattccc ctatagtgag tcgtattaat ttcgcgggat cgagatctcg atcctctacg
      601 ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg
      661 ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg
      721 gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg
      781 caccattcct tgcggcggcg gtgctcaacg gcctcnacct actactgggc tgcttcctaa
      841 tgcaggagtc gcataangga gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc
      901 tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa
      961 ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcanac cgtttcccgc
     1021 gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg
     1081 gcggagctga attacattcc caaccgcgng nannannact ggcgggnaaa cagtcgttgc
     1141 tgattggngt tgccncctnc agtctggnct gcacgcnccg ncncaaattg tcgcggnnat
     1201 taaatctcgc gcnatcaact gggnnccngc gngnngnngn nnatgnanaa cnaancgnnn
     1261 nnaancnngn nnagcngcgn gcnanancnt tnnnngcgcn acgcnnnnnn gggnngatca
     1321 tannatcgcn gnngacannn nncnntncnn ngnannntnn nnnnctannt nnnnntntnn
     1381 ntgnannnnn nnannannnn cntnancngn nnnnntttnn ccannaannn nnngngnnnn
     1441 nnnnnnnna
//
