LOCUS       sequence-65353-e        1229 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Fairhead M, Krndija D, Lowe ED, Howarth M
  TITLE     Plug-And-Play Pairing Via Defined Divalent Streptavidins.
  JOURNAL   J Mol Biol. 2013 Sep 19. pii: S0022-2836(13)00600-1. doi: 
            10.1016/j.jmb.2013.09.016.
  PUBMED    24056174
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2013 Sep 19. pii: S0022-2836(13)00600-1. doi: 
            10.1016/j.jmb.2013.09.016."
COMMENT     Sequence Label: pET21-Streptavidin-Dead Aspartate Loop
FEATURES             Location/Qualifiers
     source          1..1229
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             489..506
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(559..577)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      573..620
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      657..1014
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(744..763)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     gap             1175..1194
                     /estimated_length=20
     gap             1200..1209
                     /estimated_length=10
     gap             1211..1228
                     /estimated_length=18
ORIGIN
        1 cnnnctannn nnattttgtt taactttaag aaggagatat acatatggct gaagctggta
       61 tcaccggcac ctggtacgcc cagctgggag acaccttcat cgttaccgct ggtgctgacg
      121 gtgctctgac cggtacctac gaagccgctg ttggtgatga cgatggtgac gatgacggtg
      181 atgacgatgg agctgaatct agatacgttc tgaccggtcg ttacgactcc gctccggcta
      241 ccgacggttc cggaaccgct ctgggttgga ccgttgcttg gaaaaacaac taccgtaacg
      301 ctcactccgc taccacctgg tctggccagt acgttggtgg tgctgaagct cgtatcaaca
      361 cccagtggtt gttgacctcc ggcaccaccg aagctaacgc gtggaaatcc accctggttg
      421 gtcacgacac cttcaccaaa gttaaaccgt ccgctgcttc ctaataaaag cttgcggccg
      481 cactcgagca ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag
      541 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
      601 gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc
      661 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
      721 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
      781 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
      841 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
      901 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
      961 cttgttccna actggnnnac actcanccct atctcggtct attcttttga tttataaggg
     1021 attttgccga ttcggccnnn tggnnaaaaa atgagctgat tnncaaaaan ttnacncgaa
     1081 ntttaacnaa annnnngctt nnnnttnnng nncttttcgn nnnnnnnncn cnnnccctan
     1141 tnnntnattt nnnnaanncn nnnaannnnn ntccnnnnnn nnnnnnnnnn nnnnannctn
     1201 nnnnnnnnna nnnnnnnnnn nnnnnnnnt
//
