LOCUS       sequence-65368-e        6235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6235)
  AUTHORS   Gunzenhauser J, Olivier N, Pengo T, Manley S
  TITLE     Quantitative super-resolution imaging reveals protein stoichiometry 
            and nanoscale morphology of assembling HIV-Gag virions.
  JOURNAL   Nano Lett. 2012 Sep 12;12(9):4705-10. doi: 10.1021/nl3021076. Epub 
            2012 Aug 27.
  PUBMED    22906128
REFERENCE   2  (bases 1 to 6235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/nl3021076";
            journalName: "Nano Lett"; date: "2012-09-12- 12"; volume: "12"; 
            issue: "9"; pages: "4705-10"
COMMENT     Sequence Label: pGAGmEos2-N1
FEATURES             Location/Qualifiers
     source          1..6235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2220..2900
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    3023..3144
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3060..3079)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3114..3133
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3151..3606)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3288..3309)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3500..3519
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3633..3737
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3739..4096
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3739..3930
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3762..3782)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3947..4082
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4131..4925
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4131..4925
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4185..4204)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4795..4814
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5113..5132)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5157..5204
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5533..6121
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6022..6041
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc ggaccgatcc
      661 agcctccgcg ggcgcgcgtc gacagagaga tgggtgcgag agcgtcagta ttaagcgggg
      721 gagaattaga tcgatgggaa aaaattcggt taaggccagg gggaaagaag aagtacaagc
      781 taaagcacat cgtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      841 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      901 gatcagagga gcttcgatca ctatacaaca cagtagcaac cctctattgt gtgcaccagc
      961 ggatcgagat caaggacacc aaggaagctt tagacaagat agaggaagag caaaacaagt
     1021 ccaagaagaa ggcccagcag gcagcagctg acacaggaca cagcaatcag gtcagccaaa
     1081 attaccctat agtgcagaac atccaggggc aaatggtaca tcaggccata tcacctagaa
     1141 ctttaaatgc atgggtaaaa gtagtagaag agaaggcttt cagcccagaa gtgataccca
     1201 tgttttcagc attatcagaa ggagccaccc cacaggacct gaacacgatg ttgaacaccg
     1261 tggggggaca tcaagcagcc atgcaaatgt taaaagagac catcaatgag gaagctgcag
     1321 aatgggatag agtgcatcca gtgcatgcag ggcctattgc accaggccag ataagagaac
     1381 caaggggaag tgacatagca ggaactacta gtacccttca ggaacaaata ggatggatga
     1441 caaataatcc acctatccca gtaggagaga tctacaagag gtggataatc ctgggattga
     1501 acaagatcgt gaggatgtat agccctacca gcattctgga cataagacaa ggaccaaaag
     1561 aaccctttag agactatgta gaccggttct ataaaactct aagagctgag caagcttcac
     1621 aggaggtaaa aaattggatg acagaaacct tgttggtcca aaatgcgaac ccagattgta
     1681 agaccatcct gaaggctctc ggcccagcgg ctacactaga agaaatgatg acagcatgtc
     1741 agggagtagg aggacccggc cataaggcaa gagttttggc tgaagcaatg agccaagtaa
     1801 caaattcagc taccataatg atgcagagag gcaattttag gaaccaaaga aagattgtta
     1861 agtgtttcaa ttgtggcaaa gaagggcaca cagccagaaa ttgcagggcc cctaggaaaa
     1921 agggctgttg gaaatgtgga aaggaaggac accaaatgaa agattgtact gagagacagg
     1981 ctaatttttt agggaagatc tggccttcct acaagggaag gccagggaat tttcttcaga
     2041 gcagaccaga gccaacagcc ccaccagaag agagcttcag gtctggggta gagacaacaa
     2101 ctccccctca gaagcaggag ccgatagaca aggaactgta tcctttaact tccctcagat
     2161 cactctttgg caacgacccc tcgtcacaaa acagaaacgg ggatccaccg gtcgccacca
     2221 tgagtgcgat taagccagac atgaagatca aactccgtat ggaaggcaac gtaaacgggc
     2281 accactttgt gatcgacgga gatggtacag gcaagccttt tgagggaaaa cagagtatgg
     2341 atcttgaagt caaagagggc ggacctctgc cttttgcctt tgatatcctg accactgcat
     2401 tccattacgg caacagggta ttcgccaaat atccagacaa catacaagac tattttaagc
     2461 agtcgtttcc taaggggtat tcgtgggaac gaagcttgac tttcgaagac gggggcattt
     2521 gcattgccag aaacgacata acaatggaag gggacacttt ctataataaa gttcgatttt
     2581 atggtaccaa ctttcccgcc aatggtccag ttatgcagaa gaagacgctg aaatgggagc
     2641 cctccactga gaaaatgtat gtgcgtgatg gagtgctgac gggtgatatt catatggctt
     2701 tgttgcttga aggaaatgcc cattaccgat gtgacttcag aactacttac aaagctaagg
     2761 agaagggtgt caagttacca ggctaccact ttgtggacca ctgcattgag attttaagcc
     2821 atgacaaaga ttacaacaag gttaagctgt atgagcatgc tgttgctcat tctggattgc
     2881 ctgacaatgc cagacgataa agcggccgcg actctagatc ataatcagcc ataccacatt
     2941 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     3001 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     3061 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     3121 gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat
     3181 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     3241 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     3301 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     3361 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     3421 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     3481 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     3541 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     3601 gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     3661 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     3721 attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg
     3781 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3841 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3901 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     3961 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     4021 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     4081 cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac
     4141 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     4201 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     4261 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg
     4321 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     4381 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     4441 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     4501 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     4561 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     4621 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc
     4681 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     4741 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     4801 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     4861 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     4921 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     4981 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     5041 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag
     5101 ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc
     5161 aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg
     5221 gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg
     5281 cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc
     5341 caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga
     5401 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     5461 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5521 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5581 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5641 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     5701 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5761 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5821 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     5881 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     5941 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6001 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6061 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6121 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6181 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcat
//
