LOCUS       sequence-65405-e        4222 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of N-terminal fusions..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Kitagawa D, Vakonakis I, Olieric N, Hilbert M, Keller D, Olieric V, 
            Bortfeld M, Erat MC, Fluckiger I, Gonczy P, Steinmetz MO
  TITLE     Structural basis of the 9-fold symmetry of centrioles.
  JOURNAL   Cell. 2011 Feb 4;144(3):364-75. doi: 10.1016/j.cell.2011.01.008.
  PUBMED    21277013
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2011.01"; journalName: "Cell"; date: "2011-02-4- 4";
            volume: "144"; issue: "3"; pages: "364-75"
COMMENT     Sequence Label: pENTR Age HsSAS-6 ST
FEATURES             Location/Qualifiers
     source          1..4222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2451..2550)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2662..2681)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2673..3482
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2740..2759)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3572..4160
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4061..4080
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggcttta tcaccggtac catgagccaa
      481 gtgctgttcc accaactagt cccgttgcag gtgaaatgca aagactgtga ggagaggaga
      541 gtaagtataa gaatgagcat tgaactacaa tcagtttcta atccagttca cagaaaggac
      601 ttagttattc gtctgactga tgacacggat ccattttttt tatataacct tgttatatct
      661 gaggaagatt ttcaaagttt aaaattccag caaggtcttc tggtagactt cttagctttc
      721 ccacaaaaat ttatagatct ccttcagcaa tgtactcaag aacatgccaa agaaattcca
      781 aggtttttgc tacagttagt ttctccagca gctattttgg ataactcacc tgcattttta
      841 aatgtggtag agacaaatcc ttttaagcat cttacacacc tctcactaaa acttttacct
      901 ggaaatgatg tggagataaa gaaatttctc gcaggctgtt tgaaatgtag caaggaagaa
      961 aaattatcat tgatgcaatc actagatgat gctactaagc aactggactt tacacgaaag
     1021 acattagcag aaaaaaaaca agagctcgat aagttacgga atgaatgggc gtcacataca
     1081 gcagccttga caaacaagca ttctcaggaa ctgacaaatg aaaaggaaaa agccttgcag
     1141 gcacaggttc aatatcaaca gcagcatgaa caacagaaaa aagatttaga aatcctccat
     1201 caacaaaaca tccaccagct acaaaacaga ctgtctgagt tagaagcggc taataaagac
     1261 ttaaccgaaa gaaaatataa aggagactcc actattagag aacttaaagc aaaactttct
     1321 ggtgttgaag aggagctaca gcggactaag caagaagtcc tctctttgcg aagagagaat
     1381 tctacactag atgttgaatg ccacgagaaa gaaaagcacg ttaatcagct acaaacaaaa
     1441 gtggcagttt tagaacagga aatcaaggat aaggaccagc ttgttttaag aacaaaagag
     1501 gcatttgata caatccagga acaaaaggtg gttttagaag aaaatggtga gaaaaatcaa
     1561 gtacaactag gaaagcttga agctacaata aaatcattat ctgcagaact tctgaaggca
     1621 aatgaaatta tcaagaagtt acaaggggat ctgaaaactt taatgggtaa gttgaaattg
     1681 aagaatacag ttactattca gcaagaaaaa ctcttggctg agaaggagga aaaattacaa
     1741 aaggaacaaa aggaattaca agatgttgga cagtctcttc gaattaaaga gcaagaggta
     1801 tgcaaattac aagaacaatt agaagctaca gttaaaaaac ttgaagaaag caaacaactt
     1861 ctaaaaaata atgaaaagtt aatcacgtgg ttaaataaag aactaaatga aaatcagcta
     1921 gtgagaaagc aagatgtatt gggaccttct actactccgc ctgcacattc cagcagcaac
     1981 acaatcagaa gtggaatttc tcctaacctg aatgtggttg atggtagact gacttaccca
     2041 acctgtggga ttggttatcc tgtctcctct gcatttgcat tccagaatac cttccctcat
     2101 tcgatatctg ccaaaaatac cagccaccct ggttcaggaa caaaggttca gtttaatttg
     2161 cagtttacaa aaccaaatgc atcactagga gatgttcagt caggagcaac tattagtatg
     2221 ccttgctcaa ctgataagga aaatggtgaa aatgtagggt tggaatccaa atacctgaag
     2281 aaaagggaag atagcattcc tttacgcgga ctcagccaga acctatttag taattcagac
     2341 catcagagag atggcacttt aggagcatta catacatctt ccaaacccac agcgctcccc
     2401 tctgcgtctt cagcctattt ccctgggcag ttaccaaaca gttaatctag acccagcttt
     2461 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
     2521 cactatcagt caaaataaaa tcattatttg ccatccagct gcagctctgg cccgtgtctc
     2581 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt
     2641 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga
     2701 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
     2761 atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt
     2821 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
     2881 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
     2941 atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag
     3001 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgtccctg cgccggttgc
     3061 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
     3121 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
     3181 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt
     3241 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
     3301 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     3361 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     3421 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     3481 aatcagaatt ggttaattgg ttgtaacatt attcagattg ggccccgttc cactgagcgt
     3541 cagacccggt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     3601 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     3661 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3721 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3781 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3841 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3901 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3961 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     4021 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     4081 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     4141 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     4201 gctggccttt tgctcacatg tt
//
