LOCUS       sequence-65421-e         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-regulated lentiviral shRNA vector. Cre addition causes both EGFP
            and shRNA to be recombined out of the construct, turning OFF p53 
            shRNA expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Ventura A, Meissner A, Dillon CP, McManus M, Sharp PA, Van Parijs L,
            Jaenisch R, Jacks T
  TITLE     Cre-lox-regulated conditional RNA interference from transgenes.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Jul 13. 101(28):10380-5.
  PUBMED    15240889
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 Jul 13. 101(28):10380-5."
COMMENT     Sequence Label: pSicoR p53
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(244..277)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     335..355
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     CDS             complement(425..436)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             446..457
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     enhancer        588..891
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
       61 gacagcagag atccagtttg gttagtaccg ggcccgctct agagatccga cgcgccatct
      121 ctaggcccgc gccggccccc tcgcacagac ttgtgggaga agctcggcta ctcccctgcc
      181 ccggttaatt tgcatataat atttcctagt aactatagag gcttaatgtg cgataaaaga
      241 cagataactt cgtatagcat acattatacg aagttatcat gataggcttg gatttctata
      301 agagatacaa atactaaatt attattttaa aaaacagcac aaaaggaaac tcaccctaac
      361 tgtaaagtaa ttgtgtgttt tgagactata aatatccctt ggagaaaagc cttgtttgta
      421 ctctcctccc ctcaatttca agagaattga ggggaggaga gtactttttt ctcgagtact
      481 aggatccatt aggcggccgc gtggataacc gtattaccgc catgcattag ttattaatag
      541 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
      601 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      661 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      721 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      781 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      841 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
      901 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
      961 caccccattg acgtcaatgg
//
