LOCUS       sequence-65430-e        1778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Huang L, Jin J, Deighan P, Kiner E, McReynolds L, Lieberman J
  TITLE     Efficient and specific gene knockdown by small interfering RNAs 
            produced in bacteria.
  JOURNAL   Nat Biotechnol. 2013 Apr;31(4):350-6. doi: 10.1038/nbt.2537. Epub 
            2013 Mar 10.
  PUBMED    23475073
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.2537"; 
            journalName: "Nat Biotechnol"; date: "2013-04"; volume: "31"; issue:
            "4"; pages: "350-6"
COMMENT     Sequence Label: pGEX-4T-1-p19-T7-Vif
FEATURES             Location/Qualifiers
     source          1..1778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             523..540
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     547..566
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        547..565
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggatccatgg aacgagctat acaaggaaac gacgctaggg aacaagctaa cagtgaacgt
       61 tgggatggag gatcaggagg taccacttct cccttcaaac ttcctgacga aagtccgagt
      121 tggactgagt ggcggctaca taacgatgag acgaattcga atcaagataa tccccttggt
      181 ttcaaggaaa gctggggttt cgggaaagtt gtatttaaga gatatctcag atacgacagg
      241 acggaagctt cactgcacag agtccttgga tcttggacgg gagattcggt taactatgca
      301 gcatctcgat ttttcggttt cgaccagatc ggatgtacct atagtattcg gtttcgagga
      361 gttagtatca ccgtttctgg agggtcgcga actcttcagc atctctgtga gatggcaatt
      421 cggtctaagc aagaactgct acagcttgcc ccaatcgaag tggaaagtaa tgtatcaaga
      481 ggatgccctg aaggtactca aaccttcgaa aaagaaagcg agcaccacca ccaccaccac
      541 tgaatctaat acgactcact ataggggagc tcggaaaaca gatggcaggt gatgattgtg
      601 tggcaagtag acaggatgag gattagaaca tggaaaagtt tagtaaaaca ccatatgtat
      661 gtttcaggga aagctagggg atggttttat agacatcact atgaaagccc tcatccaaga
      721 ataagttcag aagtacacat cccactaggg gatgctagat tggtaataac aacatattgg
      781 ggtctgcata caggagaaag agactggcat ttgggtcagg gagtctccat agaatggagg
      841 aaaaagagat atagcacaca agtagaccct gaactagcag accaactaat tcatctgtat
      901 tactttgact gtttttcaga ctctgctata agaaaggcct tattaggaca catagttagc
      961 cctaggtgtg aatatcaagc aggacataac aaggtaggat ctctacaata cttggcacta
     1021 gcagcattaa taacaccaaa aaagataaag ccacctttgc ctagtgttac gaaactgaca
     1081 gaggatagat ggaacaagcc ccagaagacc aagggccaca gagggagcca cacaatgaat
     1141 ggacactagc tcgagtctag agggcccgtt taaacccgct gatcagcgtc gacctagtgt
     1201 ccattcattg tgtggctccc tctgtggccc ttggtcttct ggggcttgtt ccatctatcc
     1261 tctgtcagtt tcgtaacact aggcaaaggt ggctttatct tttttggtgt tattaatgct
     1321 gctagtgcca agtattgtag agatcctacc ttgttatgtc ctgcttgata ttcacaccta
     1381 gggctaacta tgtgtcctaa taaggccttt cttatagcag agtctgaaaa acagtcaaag
     1441 taatacagat gaattagttg gtctgctagt tcagggtcta cttgtgtgct atatctcttt
     1501 ttcctccatt ctatggagac tccctgaccc aaatgccagt ctctttctcc tgtatgcaga
     1561 ccccaatatg ttgttattac caatctagca tcccctagtg ggatgtgtac ttctgaactt
     1621 attcttggat gagggctttc atagtgatgt ctataaaacc atcccctagc tttccctgaa
     1681 acatacatat ggtgttttac taaacttttc catgttctaa tcctcatcct gtctacttgc
     1741 cacacaatca tcacctgcca tctgttttcc gcggccgc
//
