LOCUS       sequence-65431-e        1624 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Huang L, Jin J, Deighan P, Kiner E, McReynolds L, Lieberman J
  TITLE     Efficient and specific gene knockdown by small interfering RNAs 
            produced in bacteria.
  JOURNAL   Nat Biotechnol. 2013 Apr;31(4):350-6. doi: 10.1038/nbt.2537. Epub 
            2013 Mar 10.
  PUBMED    23475073
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.2537"; 
            journalName: "Nat Biotechnol"; date: "2013-04"; volume: "31"; issue:
            "4"; pages: "350-6"
COMMENT     Sequence Label: pGEX-4T-1-p19-T7-GagB500
FEATURES             Location/Qualifiers
     source          1..1624
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             523..540
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     547..566
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        547..565
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggatccatgg aacgagctat acaaggaaac gacgctaggg aacaagctaa cagtgaacgt
       61 tgggatggag gatcaggagg taccacttct cccttcaaac ttcctgacga aagtccgagt
      121 tggactgagt ggcggctaca taacgatgag acgaattcga atcaagataa tccccttggt
      181 ttcaaggaaa gctggggttt cgggaaagtt gtatttaaga gatatctcag atacgacagg
      241 acggaagctt cactgcacag agtccttgga tcttggacgg gagattcggt taactatgca
      301 gcatctcgat ttttcggttt cgaccagatc ggatgtacct atagtattcg gtttcgagga
      361 gttagtatca ccgtttctgg agggtcgcga actcttcagc atctctgtga gatggcaatt
      421 cggtctaagc aagaactgct acagcttgcc ccaatcgaag tggaaagtaa tgtatcaaga
      481 ggatgccctg aaggtactca aaccttcgaa aaagaaagcg agcaccacca ccaccaccac
      541 tgaatctaat acgactcact ataggggagc tcaagcattg ggaccagcgg ctacactaga
      601 agaaatgatg acagcatgtc agggagtagg aggacccggc cataaggcaa gagttttggc
      661 tgaagcaatg agccaagtaa caaattcagc taccataatg atgcagagag gcaattttag
      721 gaaccaaaga aagattgtta agtgtttcaa ttgtggcaaa gaagggcaca cagccagaaa
      781 ttgcagggcc cctaggaaaa agggctgttg gaaatgtgga aaggaaggac accaaatgaa
      841 agattgtact gagagacagg ctaatttttt agggaagatc tggccttcct acaagggaag
      901 gccagggaat tttcttcaga gcagaccaga gccaacagcc ccaccagaag agagcttcag
      961 gtctggggta gagacaacaa ctccccctca gaagcaggag ccgatagaca aggaactgta
     1021 tcctttaact tccctcaggt cactctttgg caacgacccc tcgtcacaat aactcgagtc
     1081 tagagggccc gtttaaaccc gctgatcagc gtcgacttat tgtgacgagg ggtcgttgcc
     1141 aaagagtgac ctgagggaag ttaaaggata cagttccttg tctatcggct cctgcttctg
     1201 agggggagtt gttgtctcta ccccagacct gaagctctct tctggtgggg ctgttggctc
     1261 tggtctgctc tgaagaaaat tccctggcct tcccttgtag gaaggccaga tcttccctaa
     1321 aaaattagcc tgtctctcag tacaatcttt catttggtgt ccttcctttc cacatttcca
     1381 acagcccttt ttcctagggg ccctgcaatt tctggctgtg tgcccttctt tgccacaatt
     1441 gaaacactta acaatctttc tttggttcct aaaattgcct ctctgcatca ttatggtagc
     1501 tgaatttgtt acttggctca ttgcttcagc caaaactctt gccttatggc cgggtcctcc
     1561 tactccctga catgctgtca tcatttcttc tagtgtagcc gctggtccca atgcttgcgg
     1621 ccgc
//
