LOCUS       sequence-65433-e       15569 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15569)
  AUTHORS   Guenthner CJ, Miyamichi K, Yang HH, Heller HC, Luo L.
  TITLE     Permanent Genetic Access to Transiently Active Neurons via TRAP: 
            Targeted Recombination in Active Populations.
  JOURNAL   Neuron. 2013 Jun 5;78(5):773-84. doi: 10.1016/j.neuron.2013.03.025.
  PUBMED    23764283
REFERENCE   2  (bases 1 to 15569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.03"; journalName: "Neuron"; date: "2013-06-5-
            5"; volume: "78"; issue: "5"; pages: "773-84"
COMMENT     Sequence Label: pArcCreER
FEATURES             Location/Qualifiers
     source          1..15569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2938..3966
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             3979..4914
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             4027..4920
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     polyA_signal    5047..5168
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5084..5103)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5138..5157
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        5276..5380
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5382..5739
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5382..5573
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5405..5425)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5590..5725
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5774..6568
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5774..6568
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5828..5847)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6438..6457
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6756..6775)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6800..6847
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        11107..11606
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(11142..11165)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     11539..11558
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             11633..12202
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphteria toxin Fragment A"
                     /label=DTA
                     /translation="DDVVILLIFCMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYDD
                     DWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKELG
                     LSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEIN
                     FETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          12279..12344
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     primer_bind     complement(12412..12429)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12418..12642
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(12710..12730)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12710..12728)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12749..12765)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12749..12765)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12762..12784)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12773..12789
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12797..12803,12804..12821,12822..12827))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12842..12863
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12980..12997)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13151..13739)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13231..13250)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13910..14701,14702..14770))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14533..14552
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14771..14875)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(14901..15356)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15038..15059)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15250..15269
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15483..15505
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15497..15514
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15498..15514
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15524..15543
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15524..15542
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aattggagcc cagggtatct ggaatcctgg ggacaaaggg ccaggactga cagggctgtg
       61 gaaccaaggt cacctggatc ttagcgctct aaagattcac ccaggcctgc cccattgctg
      121 aggacttgcg cttttcctca gaattcatct agaatctctc aggtaatttt gccatggttc
      181 ctccccatgt tctttccctg aacaggccca gcctccccac cccaccacac tgcccccatc
      241 ctaccccacc cccgccccga tccttcccca gccttccagg gggctgaagg tggcactatc
      301 tacccctcgc atgggcagga ttagtggggc gggtactgga agagtgtggg ttagaaatcc
      361 ccctctctga aggcagggag ccagccagcc tggcctcttt tccagcagca gaatgtagcc
      421 ccaggctgca gtgtgggggc gagaagggag ttgctgaggg cttctggggc gataattggg
      481 cagcgattaa atgttcccag aggagctatg gctgttgcct gtgcctgtca cctgtcactg
      541 ggctcctgtg ttcgtggctt ctgaagaccc ccggaggagg aggcagggct ccctgtgact
      601 tgtcacccca gtggggctca gagcagtgtc tcggggtcag tggcctggat ggactgccct
      661 ccatggagat aggaaatgca gtggtcagag ggtcagagca cttcagcctc ggaaacttct
      721 ccatttgtgc ccaatccccc ctacggacag tgggtggggg atcctggtca ctggccacag
      781 tgcagctggt tgacaccctt gcactctcca gcctgctcat cctcagcttc tccactctag
      841 gggactgtcc accagcttcc atgtgggtac agagagactg actctggaca ctgaagggct
      901 actgctggca tgtgtgcaga cagaccaggg tccagctgcc ccgtcaaccc taccccatgg
      961 cgtaggacct tggttaacct tcagctccct ccagactcag gcctgcagct cccggctccc
     1021 aggattcctc cataggaaaa ctctagttag tccctattct agcaatttct ctgcttgttt
     1081 cccctccctg cagtgatgcc cctctgcctg ccttctgtgt ccgcctactc acctctaatt
     1141 agaaatgcca ggagagccat tttaggggag ttttttttcc ccagaaagac attcctctca
     1201 gggatggaag ccctgggtgt gtgtgggggg ggggggaggg gggacaggtt ggctctaaga
     1261 gaggcagagc tccccaaagc ttcttgaaaa cagaggaggg ggctctgagg gatatgaggt
     1321 taggagagtt ctgtaggtgt gtgagcaaaa gtgggatggg agaagctgag atgtgagtac
     1381 acgtggatta caagtggggg ccactggtgt gtgtgtggaa ggtcatgagt ggggggtatg
     1441 agtccacaga gagggccgtg agtggggggc atgagtctac atggaagggt gtgagtgggc
     1501 taagtgctgg ttggtatgag tgttcagtgt gtatgtatga gtgtgagaga gggggagctg
     1561 gcagagagga aagggggttc ttgcaggcac catgtgtctg tgggcccctg taggcttctg
     1621 ctgtgggcag tgatatgtcc ccaagtaaca tcaggaaatg ggagacctag acctgtgttt
     1681 ctggcttggt gtaagtcaag aaggatgccc tcccccactg caccacagcg ctgttggctg
     1741 ttgccaggga atcggaaggg gcctccctct agagggaatc tcagcttccg gagccccatt
     1801 ccttatatgg catcttgctt tcctctgcag gctccttccc ctccttcctc cttctccctc
     1861 cctccttcct ccggactaac ctcttggaag gcttgagaag cttctggtcc aggtgtggct
     1921 gggtcctagg gacttcaggg ttcagcaatg ttgcctgcac atcttgatat ccctatgacc
     1981 tacccaagtg tctgtacagg ttcaagtgat acatctgttt cagggcactg cggccctgtt
     2041 cagccactga cacacagaca gggccactca gtgtccacaa acctatgaag gggtccagaa
     2101 aaagccttgc ctgagtggag ttctcgaaca atattggacc aggctaggat ttggacttat
     2161 gactcttggg gcaaggttgg gcaggaagta ggtgtgtgtg tgtgtgtgtg tgtgtgtgtg
     2221 tgtgtgtgtg tgtgtgtgtg tcccgctaac caaaggcagc tgaggataga agggtttgga
     2281 gtgagagatt cctaccaggc acttctgaag ttgcatactg tgtggtcagg atgggcaaga
     2341 gtcaggatgg gcgcatcaaa gattcagagg ggtaacatcc agctgctccc taagaaaaga
     2401 gatcatgagg ccccagggca aacagtaggc aataacctgc cttagcctca tcctcgctgc
     2461 ccaggactag gtagaggaga gtgtctccgg tccccaaaat ccctcccggt gggaggcgcg
     2521 cagcagagca cattagtcac tcggggctgt gaaggggcgg gtccttgagg gcacccacgg
     2581 gaggggagcg agtaggcgcg gaaggcgggg cctgcggcag gagagggcgc gggcgggctc
     2641 tggcgcggag cctgggcgcc gccaatggga gccagggctc cacgagctgc cgcccacggg
     2701 ccccgcgcag cataaatagc cgctggtggc ggtttcggtg cagagctcaa gcgagttctc
     2761 ccgcagccgc agtctctggg cctctctagc ttcagcggcg acgagcctgc cacactcgct
     2821 aagctcctcc ggcaccgcac acctgccact gccgctgcag ccgccggctc tgctcccttc
     2881 cggcttctgc ctcagaggag ttcttagcct gttcggagcc gcagcaccga cgaccagatg
     2941 tccaatttac tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat
     3001 gaggttcgca agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc
     3061 tggaaaatgc ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg
     3121 aaatggtttc ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc
     3181 ggtctggcag taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg
     3241 tccgggctgc cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga
     3301 aaagaaaacg ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat
     3361 ttcgaccagg ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg
     3421 gcatttctgg ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg
     3481 gttaaagata tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa
     3541 acgctggtta gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc
     3601 gagcgatgga tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg
     3661 gtcagaaaaa atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg
     3721 gaagggattt ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag
     3781 agatacctgg cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc
     3841 gctggagttt caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgtc
     3901 atgaactata tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat
     3961 ggcgatctcg agccatctgc tggagacatg agagctgcca acctttggcc aagcccgctc
     4021 atgatcaaac gctctaagaa gaacagcctg gccttgtccc tgacggccga ccagatggtc
     4081 agtgccttgt tggatgctga gccccccata ctctattccg agtatgatcc taccagaccc
     4141 ttcagtgaag cttcgatgat gggcttactg accaacctgg cagacaggga gctggttcac
     4201 atgatcaact gggcgaagag ggtgccaggc tttgtggatt tgaccctcca tgatcaggtc
     4261 caccttctag aatgtgcctg gctagagatc ctgatgattg gtctcgtctg gcgctccatg
     4321 gagcacccag tgaagctact gtttgctcct aacttgctct tggacaggaa ccagggaaaa
     4381 tgtgtagagg gcatggtgga gatcttcgac atgctgctgg ctacatcatc tcggttccgc
     4441 atgatgaatc tgcagggaga ggagtttgtg tgcctcaaat ctattatttt gcttaattct
     4501 ggagtgtaca catttctgtc cagcaccctg aagtctctgg aagagaagga ccatatccac
     4561 cgagtcctgg acaagatcac agacactttg atccacctga tggccaaggc aggcctgacc
     4621 ctgcagcagc agcaccagcg gctggcccag ctcctcctca tcctctccca catcaggcac
     4681 atgagtaaca aaggcatgga gcatctgtac agcatgaagt gcaagaacgt ggtgcccctc
     4741 tatgacctgc tgctggaggc ggcggacgcc caccgcctac atgcgcccac tagccgtgga
     4801 ggggcatccg tggaggagac ggaccaaagc cacttggcca ctgcgggctc tacttcatcg
     4861 cattccttgc aaaagtatta catcacgggg gaggcagagg gtttccctgc cacagcttga
     4921 tgagcggccg ctagataact gatcataatc agccatacca catttgtaga ggttttactt
     4981 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     5041 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     5101 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     5161 gtatcttatt taaattccgc gggtcgacga agttcctatt ccgaagttcc tattcttcaa
     5221 aaggtatagg aacttcgcta gcgattattc aggtggcact tttcggggaa atgtgcgcgg
     5281 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5341 accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag
     5401 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     5461 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     5521 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     5581 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     5641 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     5701 tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg
     5761 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     5821 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     5881 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     5941 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     6001 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     6061 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     6121 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     6181 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     6241 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     6301 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     6361 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     6421 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     6481 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     6541 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     6601 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     6661 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     6721 ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg
     6781 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg
     6841 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
     6901 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg
     6961 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt
     7021 tactcaggat catctctaga gaagttccta ttccgaagtt cctattcttc aaaaggtata
     7081 ggaacttcgc ggccgcatat ggcgcgccga gctggaccat atgaccaccg gcggcctcca
     7141 cgcctaccct gccccgcggg gtgggccggc cgccaaaccc aatgtgatcc tgcagattgg
     7201 taagtgccga gctgagatgc tggaacacgt acggaggacc caccggcatc tgttgaccga
     7261 agtgtccaag caggtggagc gagagctgaa agggttgcac aggtcggtgg gcaagctgga
     7321 gaacaacttg gacggctacg tgcccaccgg cgactcacag cgctggaaga agtccatcaa
     7381 ggcctgtctt tgccgctgcc aggagaccat cgccaacctg gagcgctggg tcaagcgtga
     7441 gatgcacgtg tggagggagg tcttctaccg gctggagagg tgggctgacc gcctggagtc
     7501 catgggcggc aaatacccag tgggcagcga gccggcccgc cacactgtct ctgtaggtgt
     7561 ggggggtcca gagccctact gccaggaagc tgatggctat gactataccg ttagccccta
     7621 tgccatcacc ccgccacctg ccgcaggaga actgcctgaa caggagtcag ttgaggctca
     7681 gcaatatcag tcttgggggc caggtgagga tgggcaaccg agccctggtg tggatacaca
     7741 gatcttcgag gacccacggg agttcctgag ccacctggaa gagtacctgc ggcaggtggg
     7801 tggctctgaa gaatattggc tgtcccagat ccagaaccac atgaatgggc cagccaagaa
     7861 gtggtgggag ttcaagcagg gctcggtgaa gaactgggtg gagttcaaga aggagtttct
     7921 gcaatacagt gagggtacac tctcccgtga agccattcag cgggagctgg agctgccgca
     7981 gaagcagggt gaaccactcg accagttcct ctggcgcaag cgggacctgt accagacact
     8041 gtatgtggac gctgaggagg aggagatcat tcagtatgtg gtgggcaccc tgcagcccaa
     8101 actcaagcgc tttctgcgcc acccactccc caagaccctg gagcagctta tccagagggg
     8161 tatggaagtg caggacggcc tggagcaggc agctgagcct tctggcaccc cactgcccac
     8221 agaggatgag acggaggcac tcacacctgc tcttaccagc gagtcagtag ccagtgacag
     8281 gacccagcct gaatagaggg gccagcccag ggtccccagc ccgcctgcca cacccagcct
     8341 gtggcttttg ccaactagga cttgagctgg ggctgactcc caaagggatg ccctgtccag
     8401 ccagacatct actcacccac tgacctggcc tgactcacaa ctgccacaca accatgctac
     8461 atggactgaa catcaagaag cccctttccc atagggctcc cacctaccgc ctacccctca
     8521 tctgtctgcc ctggtcctgg cccccagccc cattggcctc accctctaca ctctcagacc
     8581 atcacagaac acaatctggc ttcctcattc tgctccagtg tccagggccc tttgggtaat
     8641 caagaagcca agtgtctgaa aggcaacaaa aagtaggcac caaaacccag gggacatcct
     8701 agggcagatg ctaaagcaga gatgctgagg gaacctcaac ttccggggat gcggcccact
     8761 cctgcagaca cagcagatcc agctggtacc ctagctgccg cccagggcaa ccggccagtc
     8821 ttgagcagca tagctcccct cccaggggtg agctgaagcc acaaatgcag ctgaagcagc
     8881 agacctgaca tcctggcacc tcctggcccc caggtgagcc ctgcaccgtg tatcttagag
     8941 tggggggggg gcgtgcagaa ccaagtccac tttccaggag cccttcaagg cttccctagc
     9001 aaattagttg caagggtgga gtgaagtggc atcagagtgg gtgtcattgt agtgggcact
     9061 ggcacttcac ggtccaagga gggggctgag gtgggggcgg ttctggggga atttgctaag
     9121 ccagctcacc gaggaccctg tgatttctca ccccagcagt gattcatacc agtgaagaag
     9181 agcagagctc agctccgtga ctcagccatg gcaggcggag ggtccccgag gggctgagtc
     9241 ctcacaccca gccgaggcag tggctggagc ctacagagcc aggagaatga caccaggtaa
     9301 gaaagtcttg cctcctatcc tgaccaagcc tcagatccca gccactctcc agatttctgg
     9361 gttatccggg aagtcctgac aggccccagg ggtaccagct gggagcaggg taacctggtg
     9421 tccctcctgg atctagtggt gagcagttct ctcaccatct tgctgcttcc tgtgtccttc
     9481 aggtctcaag actgctgaga agtctttgct gccatgtctg gaaagggcat caccacacca
     9541 ccagcaccag cctctcttct cctgaagctg cctacacggg ttccaagaca ctttcaaggc
     9601 agagaaaaca agattacaga gaggaagtgc ctggcagggg gcaacacccc agctcagccg
     9661 gagagctgaa ggtgaagaca agccagcatg aaaccacggg tctgccatga tgcccgcccc
     9721 gctggccgct cactagctgc ctgccattag ccactgcagc ttgagcaggg tttgtaccct
     9781 tggtcctctt ggcacagagc ccagctcgct gcatggcctt tggctccccc accagaccct
     9841 tgcaggagcc ttaaggcttg ggcccctgcc cagcctgatt tttcctgctg tgccctgcct
     9901 gccgggtcaa gcccagtccc aggagacccc aggccttggc tcctagggtg ttccatgaac
     9961 ctccctgacc tgcctggtga ttgcccagct gaaacctcag ccaagcccca gctccaatta
    10021 ccttgtgctg gtagctgctt gtgtttgcag tctgagtagg ccttgttgcg gttcctccat
    10081 ctgcctggtc tattggtgtt ctgagaccag ttccactgat gttctgacag atcctccacc
    10141 ctgtgcccct gccagccccc acaagtttat ttttgcacat aagccatgac ccatactcat
    10201 ttggctggca tagggtgtgt aggtaggccc tggggactag ggagaccctg gagatctcaa
    10261 gagagtgtgg ctatccccta ttttcaccaa gccttgaata tccagccagg ctgtctgccc
    10321 ataccatctt acctcaaaga cagatatata tctatatatg attttgttaa taaaactatg
    10381 aaacttattg gagtatgtgc catttctcga gggtgtggga tgggctgaag caggacctca
    10441 gagaacactg ctcaaggagg tacaggaaca cccaccgcct atgtacccca gaaaaactag
    10501 tctagaatcc tcggccttaa aggcctcagg gacctccaaa gaccaagccc atggcctcta
    10561 ggcctggaca ggcccttgaa tccccgagct gtggtggcag gtgtgctgca ggggatctcg
    10621 tctgcagaac tgggctcgcc cagcctaccc agcctggaat ctggagacag ggtcaatgtc
    10681 ctatctaaaa gatggtgcac gccatctttc tggccttcag ttttcttatc tacaaaatga
    10741 gaaaccatct cttgactgta tccaggcctg gactcaaaaa cttggaaata acctgttaaa
    10801 ggtcacctct gtgggcagtg ctgagaggag gtactggact caaaaacttg gaaataacct
    10861 gttaaaggtc acctctgtgg gcagtactga gaggagggag aggtgaactg ccagccagac
    10921 ctcagtgagt agggctagca ggagacagag aatgctgaaa agggactatg tgtgctaggc
    10981 catatcacct tagtctgatg aagaacatgg gccgctagaa ctctagtgtc catgggaaag
    11041 catatcttgc tagcctggag gcttaattaa tcgacggtat cgataagctt gatatcgaat
    11101 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
    11161 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
    11221 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
    11281 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
    11341 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
    11401 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
    11461 gaaggggtgg gtccgggggc gggctcaggg gcgggctcas gggcggggcg ggcgcccgaa
    11521 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
    11581 cctcttcctc atctccgggc ctttcgacct gcaggtcctc gccatggatc ctgatgatgt
    11641 tgttattctt ctaatctttt gtatggaaaa cttttcttcg taccacggga ctaaacctgg
    11701 ttatgtagat tccattcaaa aaggtataca aaagccaaaa tctggtacac aaggaaatta
    11761 tgacgatgat tggaaagggt tttatagtac cgacaataaa tacgacgctg cgggatactc
    11821 tgtagataat gaaaacccgc tctctggaaa agctggaggc gtggtcaaag tgacgtatcc
    11881 aggactgacg aaggttctcg cactaaaagt ggataatgcc gaaactatta agaaagagtt
    11941 aggtttaagt ctcactgaac cgttgatgga gcaagtcgga acggaagagt ttatcaaaag
    12001 gttcggtgat ggtgcttcgc gtgtagtgct cagccttccc ttcgctgagg ggagttctag
    12061 cgttgaatat attaataact gggaacaggc gaaagcgtta agcgtagaac ttgagattaa
    12121 ttttgaaacc cgtggaaaac gtggccaaga tgcgatgtat gagtatatgg ctcaagcctg
    12181 tgcaggaaat cgtgtcaggc gatctctttg tgaaggaacc ttacttctgt ggtgtgacat
    12241 aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg
    12301 tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa
    12361 ctgatgaatg ggagcagtgg tggaatgcag atcctagagc tcgctgatca gcctcgactg
    12421 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
    12481 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
    12541 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
    12601 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
    12661 ccagctgggg ctcgacctcg agggggggcc cggtacccag cttttgttcc ctttagtgag
    12721 ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
    12781 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
    12841 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
    12901 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
    12961 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    13021 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
    13081 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
    13141 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
    13201 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
    13261 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    13321 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    13381 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    13441 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    13501 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    13561 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
    13621 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    13681 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    13741 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    13801 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
    13861 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
    13921 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
    13981 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
    14041 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
    14101 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    14161 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    14221 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    14281 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    14341 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    14401 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    14461 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    14521 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    14581 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    14641 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    14701 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    14761 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    14821 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    14881 ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
    14941 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
    15001 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
    15061 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
    15121 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
    15181 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
    15241 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
    15301 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca
    15361 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
    15421 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
    15481 ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata
    15541 gggcgaattg gagctccacc gcacgcgtc
//
