LOCUS       sequence-65435-e        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter plasmid for measuring terminator strength by inserting 
            terminator of interest between the GFP and RFP, expression driven by
            a single PBAD promoter inducible by arabinose.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Chen YJ, Liu P, Nielsen AA, Brophy JA, Clancy K, Peterson T, Voigt 
            CA
  TITLE     Characterization of 582 natural and synthetic terminators and 
            quantification of their design constraints.
  JOURNAL   Nat Methods. 2013 Jun 2. doi: 10.1038/nmeth.2515.
  PUBMED    23727987
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Jun 2. doi: 10.1038/nmeth.2515."
COMMENT     Sequence Label: pGR
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             56..67
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             74..751
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      796..867
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      883..910
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(1003..1020)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atggcatgga tgaactatac aaataataag aattcactag tagcggccgc tgcagaaaga
       61 ggagaaatac tagatggctt cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt
      121 tcgtatggaa ggttccgtta acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg
      181 tccgtacgaa ggtacccaga ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt
      241 cgcttgggac atcctgtccc cgcagttcca gtacggttcc aaagcttacg ttaaacaccc
      301 ggctgacatc ccggactacc tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt
      361 tatgaacttc gaagacggtg gtgttgttac cgttacccag gactcctccc tgcaagacgg
      421 tgagttcatc tacaaagtta aactgcgtgg taccaacttc ccgtccgacg gtccggttat
      481 gcagaaaaaa accatgggtt gggaagcttc caccgaacgt atgtacccgg aagacggtgc
      541 tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact acgacgctga
      601 agttaaaacc acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga
      661 catcaaactg gacatcacct cccacaacga agactacacc atcgttgaac agtacgaacg
      721 tgctgaaggt cgtcactcca ccggtgctta ataacgctga tagtgctagt gtagatcgct
      781 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
      841 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
      901 cctttctgcg tttatatact agagtcaatt gaggtagagg tacacacgcg aactccgata
      961 gccaattcag agtaataaac tgtgataatc aatgcaggct tcctcgctca ctgactcgct
     1021 gc
//
