LOCUS       sequence-65436-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter plasmid for measuring terminator strength by inserting 
            terminator of interest between the GFP and RFP, expression driven by
            a single PBAD promoter inducible by arabinose.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Chen YJ, Liu P, Nielsen AA, Brophy JA, Clancy K, Peterson T, Voigt 
            CA
  TITLE     Characterization of 582 natural and synthetic terminators and 
            quantification of their design constraints.
  JOURNAL   Nat Methods. 2013 Jun 2. doi: 10.1038/nmeth.2515.
  PUBMED    23727987
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Jun 2. doi: 10.1038/nmeth.2515."
COMMENT     Sequence Label: pGR
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             70..81
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             88..801
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(118..146)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     741..761
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctacctgacg ctttttatcg caactctcta ctgtttctcc ataccgtttt tttgggctag
       61 ctactagaga aagaggagaa atactagatg cgtaaaggag aagaactttt cactggagtt
      121 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
      181 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
      241 aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt
      301 gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt
      361 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa
      421 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa
      481 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac
      541 atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt
      601 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
      661 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc
      721 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat
      781 ggcatggatg aactatacaa ataataagaa ttcactagta gcggccgctg
//
