LOCUS       sequence-65448-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Chen YJ, Liu P, Nielsen AA, Brophy JA, Clancy K, Peterson T, Voigt 
            CA
  TITLE     Characterization of 582 natural and synthetic terminators and 
            quantification of their design constraints.
  JOURNAL   Nat Methods. 2013 Jun 2. doi: 10.1038/nmeth.2515.
  PUBMED    23727987
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Jun 2. doi: 10.1038/nmeth.2515."
COMMENT     Sequence Label: pGR-ECK125108723
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             133..144
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             151..828
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      873..919
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tggcatggat gaactataca aataataaga attctagcgt aaaagcaaaa cacaaatcta
       61 tccatgcaag cattcaccgc cggtttactg gcggtttttt ttcgccgtca taactagtag
      121 cggccgctgc agaaagagga gaaatactag atggcttcct ccgaagacgt tatcaaagag
      181 ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa
      241 ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa
      301 ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa
      361 gcttacgtta aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt
      421 ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac
      481 tcctccctgc aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg
      541 tccgacggtc cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg
      601 tacccggaag acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt
      661 ggtcactacg acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg
      721 ggtgcttaca aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc
      781 gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata acgctgatag
      841 tgctagtgta gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
      901 actgggcctt tcgttttatc tgttgtttgt
//
