LOCUS       sequence-65475-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  TITLE     Jackson Lab Plasmids
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pgLAP4
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             86..109
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             125..145
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             158..202
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    239..363
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(388..393,394..411,412..418)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(538..557)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tttcaggtgt cgtgaggaat tagcttggta ctaatacgac tcactatagg gagacccaag
       61 ctggctaggt aagcttggta ccatggatta caaggatgac gacgataagg caggcggggg
      121 cggggagaat ctttattttc agggcggcgg gggcgggaaa gaaaccgctg ctgctaaatt
      181 cgaacgccag cacatggaca gcggactagt ccagtgtggt ggaattctgc agatatcaac
      241 aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa
      301 ttagattttg cataaaaaac agactacata atactgtaaa acacaacata tccagtcact
      361 atggcggccg cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg
      421 tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa
      481 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
      541 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
      601 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
      661 gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg
      721 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
      781 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
      841 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
      901 ttcgtctcag ccaatccctg ggtgag
//
