LOCUS       sequence-65477-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  TITLE     Jackson Lab Plasmids
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pgLAP5
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    129..253
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(278..283,284..301,302..308)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(428..447)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tttcaggtgt cgtgaggaat tagcttggta ctaatacgac tcactatagg gagacccaag
       61 ctggctaggt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc
      121 agatatcaac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca
      181 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata
      241 tccagtcact atggcggccg cattaggcac cccaggcttt acactttatg cttccggctc
      301 gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa
      361 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
      421 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
      481 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
      541 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg
      601 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
      661 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
      721 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
      781 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
      841 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
      901 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttg
//
