LOCUS       sequence-65478-e         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  TITLE     Jackson Lab Plasmids
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pgLAP5
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..77
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(115..132)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    121..345
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(478..497)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    629..676
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 ctcttggcat ggacgagctg tacaagtaat tcgaaggtaa gcctatccct aaccctctcc
       61 tcggtctcga ttctacgcgt accggttagt aatgagttta aacccgctga tcagcctcga
      121 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
      181 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
      241 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
      301 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
      361 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
      421 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
      481 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
      541 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
      601 ttgattaggg tgatggttca cgtacctaga agttcctatt ccgaagttcc tattctctag
      661 aaagtatagg aacttccttg gccaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
      721 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
      781 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
      841 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
      901 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc
      961 gtgcacaggg tgtc
//
