LOCUS       sequence-65495-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Heaton JH, Dlakic WM, Dlakic M, Gelehrter TD
  TITLE     Identification and cDNA cloning of a novel RNA-binding protein that 
            interacts with the cyclic nucleotide-responsive sequence in the 
            Type-1 plasminogen activator inhibitor mRNA.
  JOURNAL   J Biol Chem. 2001 Feb 2;276(5):3341-7. Epub 2000 Sep 22.
  PUBMED    11001948
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2001-02-2"; volume: "276(5)"; pages: "3341-7. Epub 
            2000 Sep 22"
COMMENT     Sequence Label: pET-15b PAI-RBP
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    69..93
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             108..130
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             149..166
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             176..193
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc
       61 actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt gtttaacttt
      121 aagaaggaga tataccatgg gcagcagcca tcatcatcat catcacagca gcggcctggt
      181 gccgcgcggc agccatatgc ctgggcactt acaggaaggc ttcggctgcg tggtcaccaa
      241 ccgattcgac cagttatttg acgacgaatc ggaccccttc gaggtgctga aggcagcaga
      301 gaacaagaaa aaagaagccg gcgggggcgg cgttgggggc cctggggcca agagcgcagc
      361 tcaggccgcg gcccagacca actccaacgc ggcaggcaaa cagctgcgca aggagtccca
      421 gaaagaccgc aagaacccgc tgccccccag cgttggcgtg gttgacaaga aagaggagac
      481 gcagccgccc gtggcgctta agaaagaagg aataagacga gttggaagaa gacctgatca
      541 acaacttcag ggtgaaggga aaataattga tagaagacca gaaaggcgac cacctcgtga
      601 acgaagattc gaaaagccac ttgaagaaaa gggtgaagga ggcgaatttt cagttgatag
      661 accgattatt gaccgaccta ttcgaggtcg tggtggtctt ggaagaggtc gagggggccg
      721 tggacgtgga atgggccgag gagatggatt tgattctcgt ggcaaacgtg aatttgatag
      781 gcatagtgga agtgatagat ctggcctgaa gcacgaggac aaacgtggag gtagcggatc
//
