LOCUS       sequence-65502-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Heaton JH, Dlakic WM, Dlakic M, Gelehrter TD
  TITLE     Identification and cDNA cloning of a novel RNA-binding protein that 
            interacts with the cyclic nucleotide-responsive sequence in the 
            Type-1 plasminogen activator inhibitor mRNA.
  JOURNAL   J Biol Chem. 2001 Feb 2;276(5):3341-7. Epub 2000 Sep 22.
  PUBMED    11001948
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2001-02-2"; volume: "276(5)"; pages: "3341-7. Epub 
            2000 Sep 22"
COMMENT     Sequence Label: pCELF PAI-RBP v2
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             66..719
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     677..699
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             726..743
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttggcgaagc ttcgccaatt ctctagagat ccctcgacct cgagatccat tgtgctggat
       61 ctacaatggc ccctatacta ggttattgga aaattaaggg ccttgtgcaa cccactcgac
      121 ttcttttgga atatcttgaa gaaaaatatg aagagcattt gtatgagcgc gatgaaggtg
      181 ataaatggcg aaacaaaaag tttgaattgg gtttggagtt tcccaatctt ccttattata
      241 ttgatggtga tgttaaatta acacagtcta tggccatcat acgttatata gctgacaagc
      301 acaacatgtt gggtggttgt ccaaaagagc gtgcagagat ttcaatgctt gaaggagcgg
      361 ttttggatat tagatacggt gtttcgagaa ttgcatatag taaagacttt gaaactctca
      421 aagttgattt tcttagcaag ctacctgaaa tgctgaaaat gttcgaagat cgtttatgtc
      481 ataaaacata tttaaatggt gatcatgtaa cccatcctga cttcatgttg tatgacgctc
      541 ttgatgttgt tttatacatg gacccaatgt gcctggatgc gttcccaaaa ttagtttgtt
      601 ttaaaaaacg tattgaagct atcccacaaa ttgataagta cttgaaatcc agcaagtata
      661 tagcatggcc tttgcagggc tggcaagcca cgtttggtgg tggcgaccat cctccaaaat
      721 cggatctggt tccgcgtgga tccatgcctg ggcacttaca ggaaggcttc ggctgcgtgg
      781 tcaccaaccg attcgaccag ctatttgacg acgaatcgga ccctttcgag gtactgaagg
      841 cagcagaga
//
