LOCUS       sequence-65504-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Heaton JH, Dlakic WM, Dlakic M, Gelehrter TD
  TITLE     Identification and cDNA cloning of a novel RNA-binding protein that 
            interacts with the cyclic nucleotide-responsive sequence in the 
            Type-1 plasminogen activator inhibitor mRNA.
  JOURNAL   J Biol Chem. 2001 Feb 2;276(5):3341-7. Epub 2000 Sep 22.
  PUBMED    11001948
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2001-02-2"; volume: "276(5)"; pages: "3341-7. Epub 
            2000 Sep 22"
COMMENT     Sequence Label: pCELF PAI-RBP v3
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             67..720
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     678..700
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             727..744
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 attggcgaag cttcgccaat tctctagaga tccctcgacc tcgagatcca ttgtgctgga
       61 tctacaatgg cccctatact aggttattgg aaaattaagg gccttgtgca acccactcga
      121 cttcttttgg aatatcttga agaaaaatat gaagagcatt tgtatgagcg cgatgaaggt
      181 gataaatggc gaaacaaaaa gtttgaattg ggtttggagt ttcccaatct tccttattat
      241 attgatggtg atgttaaatt aacacagtct atggccatca tacgttatat agctgacaag
      301 cacaacatgt tgggtggttg tccaaaagag cgtgcagaga tttcaatgct tgaaggagcg
      361 gttttggata ttagatacgg tgtttcgaga attgcatata gtaaagactt tgaaactctc
      421 aaagttgatt ttcttagcaa gctacctgaa atgctgaaaa tgttcgaaga tcgtttatgt
      481 cataaaacat atttaaatgg tgatcatgta acccatcctg acttcatgtt gtatgacgct
      541 cttgatgttg ttttatacat ggacccaatg tgcctggatg cgttcccaaa attagtttgt
      601 tttaaaaaac gtattgaagc tatcccacaa attgataagt acttgaaatc cagcaagtat
      661 atagcatggc ctttgcaggg ctggcaagcc acgtttggtg gtggcgacca tcctccaaaa
      721 tcggatctgg ttccgcgtgg atccatgcct gggcacttac aggaaggctt cggctgcgtg
      781 gtcaccaatc gattcgacca gctatttgac gacgaatcg
//
