LOCUS       sequence-65512-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Codon optimized Cas9_SV40 NLS with intron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Friedland AE, Tzur YB, Esvelt KM, Colaiacovo MP, Church GM, Calarco 
            JA
  TITLE     Heritable genome editing in C. elegans via a CRISPR-Cas9 system.
  JOURNAL   Nat Methods. 2013 Jun 30. doi: 10.1038/nmeth.2532.
  PUBMED    23817069
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Jun 30. doi: 10.1038/nmeth.2532."
COMMENT     Sequence Label: Peft-3::cas9-SV40_NLS::tbb-2 3'UTR
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcgatgacga tgttctctgg cttgtgacgt cccatgacct tgacgagctc gtcgacgacc
       61 ttgacggtct ggaggattcc cttcttgatg gctggggatc cggcgaggtt ggcgatgtgc
      121 tcgtggaggg agtctccctg tccggagacc tgggccttct ggatgtcctc cttgaaggtg
      181 agggagtcgt cgtggatgag ctgcatgaac tgaaaattta aatcagatta gttagttagt
      241 aaaatcatgt ttaaacttac gttacggttg gcgaatccgt cggacttgag gaagtcgagg
      301 atggtctttc cggactgctt gtcacggatt ccgttgatga gcttacggga gagacgtccc
      361 catccggtgt aacgacgacg cttgagctgc ttcatgacct tgtcgtcgaa gaggtgggcg
      421 taggtcttga gacgctcctc gatcatctca cggtcctcga agagggtgag ggtgaggacg
      481 atgtcctcga ggatgtcctc gttctcctcg ttgtcgagga agtccttgtc cttgatgatc
      541 ttgaggaggt cgtggtaggt tccgagggag gcgttgaaac ggtcctcgac tccggagatc
      601 tcgacggagt cgaagcactc gatcttcttg aagtagtcct ccttgagctg cttgacggtg
      661 accttacggt tggtcttgaa gaggaggtcg acgatggcct tcttctgctc tccggagagg
      721 aaggctggct tacgcattcc ctcggtgacg tacttgacct tggtgagctc gttgtagacg
      781 gtgaagtact cgtagaggag ggagtgcttt gggaggacct tctcgtttgg gaggttcttg
      841 tcgaagttgg tcatacgctc gatgaaggac tgggcggagg ctcccttgtc gacgacctcc
      901 tcgaagttcc atg
//
